Small quinolinium-based enzymatic probes via blue-to-red ratiometric fluorescence

The Analyst
Pan WangGuoqing Zhang

Abstract

A small fluorescence ratiometric probe consisting of a single dye species, N-methyl-6-hydroxyquinolinium (MHQ), and coupled enzymatic substrates, exhibits a dramatic colour change (deep blue to red) and possesses a huge response ratio (over 2000 fold) upon specific recognition of target enzymes. Such dramatic responses are attributed to the excited-state proton transfer processes of MHQ molecules in water. Here the detection of β-galactosidase and porcine pancreatic lipase is successfully demonstrated and this class of molecules has the potential to be developed as a "naked-eye" probe in vitro.

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Citations

Jun 20, 2017·Journal of the American Chemical Society·Beatriz Lozano-TorresFélix Sancenón
May 31, 2017·Angewandte Chemie·Stella SchmodeRalf Ludwig
Jul 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuanyuan HuangZhaosheng Qian
Apr 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tumpa Gorai, Uday Maitra
Feb 20, 2021·Analytical and Bioanalytical Chemistry·Beatriz Lozano-TorresRamón Martínez-Máñez

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