Small Rho GTPases and the Effector VipA Mediate the Invasion of Epithelial Cells by Filamentous Legionella pneumophila

Frontiers in Cellular and Infection Microbiology
Akriti PrasharMauricio R Terebiznik

Abstract

Legionella pneumophila (Lp) exhibits different morphologies with varying degrees of virulence. Despite their detection in environmental sources of outbreaks and in respiratory tract secretions and lung autopsies from patients, the filamentous morphotype of Lp remains poorly studied. We previously demonstrated that filamentous Lp invades lung epithelial cells (LECs) and replicates intracellularly in a Legionella containing vacuole. Filamentous Lp activates β1integrin and E-cadherin receptors at the surface of LECs leading to the formation of actin-rich cell membrane structures we termed hooks and membrane wraps. These structures entrap segments of an Lp filament on host cell surface and mediate bacterial internalization. Here we investigated the molecular mechanisms responsible for the actin rearrangements needed for the formation and elongation of these membrane wraps and bacterial internalization. We combined genetic and pharmacological approaches to assess the contribution of signaling downstream of β1integrin and E-cadherin receptors, and Lp Dot/Icm secretion system- translocated effectors toward the invasion process. Our studies demonstrate a multi-stage mechanism of LEC invasion by filamentous Lp. Bacterial attachment to h...Continue Reading

References

Dec 1, 1979·Journal of Clinical Pathology·F G Rodgers
Mar 1, 1981·The Journal of Infectious Diseases·J A DaisyH M Friedman
Apr 1, 1980·American Journal of Clinical Pathology·F J HernandezP H Edelstein
Dec 8, 1995·The Journal of Biological Chemistry·P D BurbeloA Hall
Apr 1, 1995·Current Microbiology·N P CianciottoD W Kamp
Mar 1, 1995·The American Journal of Physiology·D C GruenertJ H Widdicombe
May 1, 1993·The Journal of Infectious Diseases·C H ModyG B Toews
Jul 1, 1996·Trends in Microbiology·B S Fields
Oct 19, 2000·Methods in Enzymology·S NarumiyaM Uehata
Feb 24, 2001·Cellular Microbiology·G MolinariG S Chhatwal
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·L A KnodlerB B Finlay
Nov 28, 2001·Molecular Microbiology·H HilbiH A Shuman
Jun 19, 2002·FEMS Microbiology Reviews·Michael SteinertJörg Hacker
Jul 9, 2002·Genes & Development·Anja Schmidt, Alan Hall
Mar 15, 2003·Science·Aaron F StraightTimothy J Mitchison
Nov 5, 2003·Current Topics in Microbiology and Immunology·P HilpeläP Lappalainen
Apr 10, 2004·Science·Pascale Cossart, Philippe J Sansonetti
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuan GaoYi Zheng
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·D R CliftonT Hackstadt
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Wenjun Guo, Filippo G Giancotti
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Satyajit K MitraDavid D Schlaepfer
Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nadim ShohdyHoward A Shuman
Jun 29, 2005·Current Topics in Microbiology and Immunology·B B Finlay
Jun 29, 2005·Current Topics in Microbiology and Immunology·M C Schlumberger, W D Hardt
Feb 1, 2006·PLoS Pathogens·Mickey PentecostManuel R Amieva
Feb 8, 2006·Applied and Environmental Microbiology·Zhenyu PiaoShin-ichi Yoshida
Sep 23, 2006·Nature Reviews. Molecular Cell Biology·Erin D Goley, Matthew D Welch

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Citations

Feb 23, 2020·Cellular Microbiology·Sudha MoorthyEric A Klein

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Methods Mentioned

BETA
lavage
GTPases
GTPase
PCR
fluorescence microscopy
nucleotide exchange

Software Mentioned

ImageJ
Graph Pad Prism
Adobe Photoshop Illustrator
MetaMorph
Volocity

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