Small Rho GTPases are important for acinus formation in a human salivary gland cell line

Cell and Tissue Research
Virgínia Oliveira CremaMarinilce F Santos

Abstract

Rho GTPases participate in a wide variety of signal transduction pathways regulating the actin cytoskeleton, gene expression, cellular migration and proliferation. The aim of this study was to evaluate the role of Rho GTPases in signal transduction pathways during acinus formation in a human salivary gland (HSG) cell line initiated by extracellular matrix (ECM; Matrigel) alone or in combination with epidermal growth factor, basic fibroblast growth factor and lysophosphatidic acid (LPA). Immunohistochemical and Western blotting analyses showed that HSG cells contained RhoA, RhoB, Rac1 and Cdc42 proteins. All growth factors enhanced the effects of ECM on acinus formation, in a pathway dependent on PI3-kinase and Rho GTPases. The role of ROCK, a major RhoA effector, seemed limited to cortical actin polymerization. LPA stimulated cell migration and acinus formation in a PI3-kinase-independent pathway. The results suggest that Rho proteins are important for epithelial-mesenchymal interactions during salivary gland development.

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Citations

Sep 12, 2008·Journal of Molecular Histology·Virgínia O CremaMarinilce F Santos
Jul 18, 2012·Journal of Molecular Histology·Hiroshi ShiratsuchiYoshiyuki Yonehara
Jun 2, 2007·Journal of Cellular Biochemistry·Vanda SzlávikGábor Varga
Mar 12, 2015·Orphanet Journal of Rare Diseases·Karin Soares CunhaEliane Pedra Dias
Feb 4, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·John M PrinsKathleen M George
Dec 17, 2014·Experimental & Molecular Medicine·Sung-Min HwangKyungpyo Park
Jun 14, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Wen Yu WongKirsten H Limesand
Apr 25, 2014·American Journal of Physiology. Cell Physiology·Farid G El-SayedGary A Weisman
Jun 18, 2019·Expert Opinion on Investigational Drugs·Sarah PringleFrans G M Kroese

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