Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance

Molecular Plant Pathology
Mark DerbyshireSylvain Raffaele

Abstract

Fungal plant pathogens secrete effector proteins and metabolites to cause disease. Additionally, some species transfer small RNAs (sRNAs) into plant cells to silence host mRNAs through complementary base pairing and suppress plant immunity. The fungus Sclerotinia sclerotiorum infects over 600 plant species, but little is known about the molecular processes that govern interactions with its many hosts. In particular, evidence for the production of sRNAs by S. sclerotiorum during infection is lacking. We sequenced sRNAs produced by S. sclerotiorum in vitro and during infection of two host species, Arabidopsis thaliana and Phaseolus vulgaris. We found that S. sclerotiorum produces at least 374 distinct highly abundant sRNAs during infection, mostly originating from repeat-rich plastic genomic regions. We predicted the targets of these sRNAs in A. thaliana and found that these genes were significantly more down-regulated during infection than the rest of the genome. Predicted targets of S. sclerotiorum sRNAs in A. thaliana were enriched for functional domains associated with plant immunity and were more strongly associated with quantitative disease resistance in a genome-wide association study (GWAS) than the rest of the genome. Mu...Continue Reading

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Citations

Oct 28, 2020·Molecular Plant-microbe Interactions : MPMI·Jeanne M HarrisMaria Elena Alvarez
Jun 22, 2021·Frontiers in Plant Science·Dandan ShaoMitchell G Roth
Sep 15, 2020·Microbiological Research·Manisha PhourBernard R Glick

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Datasets Mentioned

BETA
SRP151049
GSE106811
SRX1705129

Methods Mentioned

BETA
Illumina sequencing

Software Mentioned

Python
CoLIde
R bioconductor package
Bedtools
TopGO
REPET
Rfam
psRNATarget

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