May 10, 2017

Small teams of myosin Vc motors coordinate their stepping for efficient cargo transport on actin bundles

The Journal of Biological Chemistry
Elena B KrementsovaM Yusuf Ali

Abstract

Myosin Vc (myoVc) is unique among vertebrate class V myosin isoforms in that it requires teams of motors to move continuously on single actin filaments. Single molecules of myoVc cannot take multiple hand-over-hand steps from one actin-binding site to the next without dissociating, in stark contrast to the well studied myosin Va (myoVa) isoform. At low salt, single myoVc motors can, however, move processively on actin bundles, and at physiologic ionic strength, even teams of myoVc motors require actin bundles to sustain continuous motion. Here, we linked defined numbers of myoVc or myoVa molecules to DNA nanostructures as synthetic cargos. Using total internal reflectance fluorescence microscopy, we compared the stepping behavior of myoVc versus myoVa ensembles and myoVc stepping patterns on single actin filaments versus actin bundles. Run lengths of both myoVc and myoVa teams increased with motor number, but only multiple myoVc motors showed a run-length enhancement on actin bundles compared with actin filaments. By resolving the stepping behavior of individual myoVc motors with a quantum dot bound to the motor domain, we found that coupling of two myoVc motors significantly decreased the futile back and side steps that were f...Continue Reading

  • References38
  • Citations3

References

  • References38
  • Citations3

Citations

Mentioned in this Paper

Vertebrates
Study
Patterns
Gait, Drop Foot
Myo5a protein, mouse
Actins
Neurons
MYO5C
MYO5A
Sustain

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