Small-volume on-line sensor for continuous measurement of gamma-aminobutyric acid

Analytical Chemistry
O NiwaK Torimitsu

Abstract

We report the first on-line electrochemical sensor for the continuous measurement of gamma-aminobutyric acid (GABA), which is a well-known inhibitory neurotransmitter in the nervous system. The sensor is composed of a glutamate oxidase (GluOx) and catalase immobilized small-volume enzymatic reactor and a glassy carbon (GC) electrode modified with a top layer film consisting of gabase and GluOx coimmobilized bovine serum albumin and an Ospoly(vinylpyrridine) bottom layer film containing horseradish peroxidase. The response of the sensor depends on the alpha-ketoglutarate concentration and is almost saturated when its concentration is 100 times higher than GABA. The sensor exhibits a sensitivity of 1.56 nA/microM for GABA under optimized conditions and shows almost no response when 10 microM glutamate is continuously injected. A detection limit of 0.1 microM is obtained with a linear range of 0.1-10 microM. GABA can be measured in the absence of alpha-ketoglutarate when there is L-glutamate in the sample solution, which is a typical condition for the extracellular measurement of cultured nerve cells.

Citations

Feb 24, 2017·Journal of the Science of Food and Agriculture·Nooshin NikmaramStuart K Johnson
Jan 13, 2012·Accounts of Chemical Research·Meining ZhangLanqun Mao
Oct 26, 2012·Journal of the American Chemical Society·Anastasiya MasharinaKai Johnsson
Jan 21, 2017·Analytical Chemistry·Tongfang XiaoLanqun Mao

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