Abstract
Yacon, Smallanthus sonchifolius, has anti-hypertensive, anti-inflammatory, and anti-cancer potential. However, its neuroprotective and anti-neuroinflammatory effects are unknown. Moreover, activation of microglia has been considered a mechanism in the development of Alzheimer's disease. Therefore, the aim of this study was to determine the neuroprotective effects of an ethanolic yacon leaf extract (YLE) on lipopolysaccharide (LPS)-induced neuroinflammation in vitro and in vivo. The viability of microglial BV2 cells was tested with 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolim-5-carboxanilide. The production of nitric oxide (NO) was determined by the Griess reagent. mRNA expression and protein levels of inflammatory mediators were evaluated by the real-time polymerase chain reaction and immunohistochemistry, respectively. In addition, we performed histological analysis in mice treated with an intraperitoneal injection of LPS (250 μg/kg). Our results showed that treatment with YLE significantly reduced NO production in LPS-stimulated BV2 cells. YLE also decreased mRNA levels of the inflammatory factors tumor necrosis factor alpha, inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-1 beta. In vivo, YLE (...Continue Reading
References
Apr 10, 1986·The New England Journal of Medicine·R Katzman
Feb 22, 2001·Journal of Neurochemistry·R C ChangJ S Hong
Apr 18, 2001·Brain Research Bulletin·N P TurrinC R Plata-Salamán
Jul 17, 2001·Microscopy Research and Technique·M E Bamberger, G E Landreth
Jul 6, 2002·Journal of Neuroimmunology·Bei Ping HeMichael J Strong
Feb 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Belén G RamírezMaría L de Ceballos
Mar 15, 2005·Journal of Neuroinflammation·Nikunj S PatelMichael J Mullan
Jan 5, 2007·Glia·Liya QinFulton T Crews
Jun 7, 2007·Nature Reviews. Neuroscience·Frank M LaFerlaSalvatore Oddo
Apr 19, 2008·Brain : a Journal of Neurology·Jens SchmidtMarinos C Dalakas
Jun 24, 2008·Nature Reviews. Neuroscience·Fernando Gómez-Pinilla
Sep 19, 2008·Expert Opinion on Drug Metabolism & Toxicology·Gaylia Jean Harry, Andrew D Kraft
Jan 12, 2010·Journal of Alzheimer's Disease : JAD·M Paul Murphy, Harry LeVine
Feb 4, 2010·Alzheimer's Research & Therapy·Elina ZotovaDelphine Boche
Feb 5, 2010·British Journal of Pharmacology·Won-Kyo JungIl-Whan Choi
Oct 19, 2010·Journal of Ethnopharmacology·Rejane Barbosa de OliveiraFernando Batista Da Costa
Oct 22, 2010·Experimental & Molecular Medicine·Hey-Kyeong JeongEun-hye Joe
Jan 5, 2011·Cell Metabolism·Rebecca G BakerSankar Ghosh
May 12, 2012·International Journal of Alzheimer's Disease·Thomas O ObisesanJohn Kwagyan
Apr 19, 2015·Journal of Neuroinflammation·Amir NazemYousef Al-Abed
Nov 6, 2015·Journal of Medicinal Food·Kang Pa LeeHeeok Hong
Jul 28, 2016·Nutrients·Brunno F R CaetanoLuís F Barbisan
Feb 6, 2018·Nutrition Research and Practice·Kang Pa LeeDea Won Lee