Smartphone-based colorimetric assay of antioxidants in red wine using oxidase-mimic MnO2 nanosheets

The Analyst
Yongfei LiYi Wang

Abstract

In this work, we report a fast and colorimetric method for the detection of antioxidants based on the oxidase-like activity of two-dimensional MnO2 nanosheets. Specifically, MnO2 nanosheets can transform the colourless substrate, 3,3',5,5'-tetramethylbenzidine (TMB), into a deep blue product (oxTMB) via catalytic oxidation, with a specific absorption peak arising at 652 nm. In the presence of antioxidants, both the dramatic colour fading and the decrease in absorbance at 652 nm are observed owing to the inhibition of catalytic oxidation of TMB, where MnO2 nanosheets can be transformed into Mn2+ with the aid of antioxidants. Based on the linear relationship between the absorbance at 652 nm and the concentration of the antioxidants, quantitative determination of antioxidants in the range of 0.1-12 μM has been achieved. Moreover, combined with smartphone-based technology, a portable colorimetric analytical platform for assay of antioxidants is further developed. This method shows advantages including easy operation, low cost, rapid detection and high sensitivity and has been successfully applied in the determination of total antioxidants in red wine.

References

Sep 1, 1991·Circulation·D Steinberg
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Oct 22, 2002·Expert Opinion on Investigational Drugs·Bernd Moosmann, Christian Behl
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Dejian HuangRonald L Prior
May 12, 2005·Journal of Agricultural and Food Chemistry·Ronald L PriorKaren Schaich
Jan 25, 2006·Chemico-biological Interactions·M ValkoM Mazur
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Apr 1, 2008·Analytica Chimica Acta·Luís M MagalhãesJosé L F C Lima
Jul 26, 2008·Nature Nanotechnology·Lizeng GaoXiyun Yan
Nov 4, 2008·Journal of the American Chemical Society·Kazuya KaiJun Kawamata
Aug 19, 2010·Oxidative Medicine and Cellular Longevity·Kanti Bhooshan Pandey, Syed Ibrahim Rizvi
Nov 16, 2010·Critical Reviews in Biotechnology·C M AjilaJ R Valéro
Jun 23, 2012·Chemistry and Physics of Lipids·I PinchukD Lichtenberg
Oct 19, 2013·Bulletin of Experimental Biology and Medicine·E B MenshchikovaN V Kandalintseva
Jun 12, 2016·Analytica Chimica Acta·Vasiliki GatselouDimosthenis L Giokas
Feb 28, 2017·Analytical Chemistry·Zhuangqiang GaoXiaohu Xia
Sep 1, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Liuying HeYueying Liu
Sep 29, 2018·Biosensors & Bioelectronics·Yi WangYingnan Zhao
Apr 8, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Juan ChenYuehe Lin

❮ Previous
Next ❯

Citations

Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuanqiang HaoShu Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
antioxidant

Software Mentioned

Colour Pickers

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.