Smartphone-based mobile digital PCR device for DNA quantitative analysis with high accuracy

Biosensors & Bioelectronics
Tong GouYing Mu

Abstract

Digital polymerase chain reaction (dPCR) circumventing the external calibration and potentially providing absolute quantification of nucleic acids has become an increasingly popular manifestation of PCR in biological researches. However, currently reported or commercial dPCR devices are not suitable for applications in laboratories or zones with limited infrastructures, due to low function integration, cost-inefficiency, or weak mobility. Herein, in order to enable accurate DNA quantitative analysis in such situations, we have developed a smartphone-based mobile dPCR device integrated with thermal cycling control, on-chip dPCR, data acquisition, and result analysis. All the function units are automatically controlled using a customized Android software. The device is approximately 90 mm × 90 mm × 100 mm in size and about 500 g in weight, only costing about 320 dollars except the smartphone. Coupled with the self-priming dPCR chip previously developed by our lab, the device is able to accurately quantify down to 10 copies of the human 18 S ribosomal DNA fragment inserted in a plasmid. Comparing to the commercial QuantStudio™ 3D dPCR platform, our device achieves a comparable analytical accuracy. Besides, our device is capable of...Continue Reading

Citations

Mar 7, 2020·The Analyst·Wenhao ZhaoJinhong Guo
Aug 21, 2020·BioTechniques·Hanliang ZhuPavel Neužil
Aug 28, 2020·Lab on a Chip·Christian D AhrbergBong Geun Chung
Dec 10, 2020·Science China. Chemistry·Yongxi ZhaoChunhai Fan
Dec 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kangning WangWenming Wu
Mar 12, 2021·Lab on a Chip·Qi SongWeijia Wen
Mar 16, 2021·Advanced Science·Joon Soo ParkTza-Huei Wang
Mar 20, 2021·Biosensors & Bioelectronics·Martina GaňováPavel Neužil
Apr 29, 2021·Lab on a Chip·Mengcheng JiangYanyi Huang
Sep 19, 2020·Analytical Chemistry·Ahmad MoniriJesus Rodriguez-Manzano
Nov 16, 2018·Analytical Chemistry·Xiong DingChangchun Liu
Dec 4, 2021·Scientific Reports·Abbas JaliliAmir Hossein Kazemipour Ashkezari

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