Smartphone-based rapid quantification of viable bacteria by single-cell microdroplet turbidity imaging

The Analyst
Xiaonan CuiBo Ma

Abstract

Standard plate count (SPC) has been recognized as the golden standard for the quantification of viable bacteria. However, SPC usually takes one to several days to grow individual cells into a visible colony, which greatly hampers its application in rapid bacteria enumeration. Here we present a microdroplet turbidity imaging based digital standard plate count (dSPC) method to overcome this hurdle. Instead of cultivating on agar plates, bacteria are encapsulated in monodisperse microdroplets for single-cell cultivation. Proliferation of the encapsulated bacterial cell produced a detectable change in microdroplet turbidity, which allowed, after just a few bacterial doubling cycles (i.e., a few hours), enumeration of viable bacteria by visible-light imaging. Furthermore, a dSPC platform integrating a power-free droplet generator with smartphone-based turbidity imaging was established. As proof-of-concept demonstrations, a series of Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Bacillus subtilis) samples were quantified via the smartphone dSPC accurately within 6 hours, representing a detection sensitivity of 100 CFU ml-1 and at least 3 times faster. In addition, Enterobacter sakazakii (E. sakazakii) in infan...Continue Reading

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Citations

Feb 1, 2019·Lab on a Chip·Shuaiguo ZhaoTony Jun Huang
Sep 9, 2020·Lab on a Chip·Fengyi LiuBo Ma
Aug 8, 2020·Frontiers in Cellular and Infection Microbiology·Antti VasalaOlli H Laitinen
Nov 10, 2020·Analytical and Bioanalytical Chemistry·Binghan LiJiao Yang

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Methods Mentioned

BETA
light-scattering
Chip
biopsy
chips

Software Mentioned

Windows
CDroplet
dSPC
SPSS
dSPC app

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