SMb20651 is another acyl carrier protein from Sinorhizobium meliloti

Microbiology
Ana Laura Ramos-VegaI M López-Lara

Abstract

Acyl carrier proteins (ACPs) are small acidic proteins that carry growing acyl chains during fatty acid or polyketide synthesis. In rhizobia, there are four different and well-characterized ACPs: AcpP, NodF, AcpXL and RkpF. The genome sequence of Sinorhizobium meliloti 1021 reveals two additional ORFs that possibly encode additional ACPs. One of these, smb20651, is located on the plasmid pSymB as part of an operon. The genes of the operon encode a putative asparagine synthetase (AsnB), the predicted ACP (SMb20651), a putative long-chain fatty acyl-CoA ligase (SMb20650) and a putative ammonium-dependent NAD+ synthetase (NadE1). When SMb20651 was overexpressed in Escherichia coli, [3H]beta-alanine, a biosynthetic building block of 4'-phosphopantetheine, was incorporated into the protein in vivo. The purified SMb20651 was modified with 4'-phosphopantetheine in the presence of S. meliloti holo-ACP synthase (AcpS). Also, holo-SMb20651 was modified in vitro with a malonyl group by malonyl CoA-ACP transacylase. In E. coli, coexpression of SMb20651 together with other proteins such as AcpS and SMb20650 led to the formation of additional forms of SMb20651. In this bacterium, acylation of SMb20651 with C12 : 0 or C18 : 0 fatty acids was ...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jan 1, 1972·Methods in Enzymology·R H Burris
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Dec 1, 1970·The Journal of Applied Bacteriology·M T Sherwood
Jul 1, 1984·Journal of Bacteriology·T M FinanE R Signer
Nov 1, 1996·Chemistry & Biology·R H LambalotC T Walsh
Dec 13, 1996·The Journal of Biological Chemistry·K A BrozekC R Raetz
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M OsteråsD Le Rudulier
Jan 8, 2000·The Journal of Biological Chemistry·R S FlugelC T Walsh
Mar 29, 2000·Biochemical and Biophysical Research Communications·S S KhandekarJ Lonsdale
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·I M López-Lara, O Geiger
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Clay Fuqua, E Peter Greenberg
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, James Baddiley
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Anke BeckerJacques Batut
Oct 6, 2005·Applied and Environmental Microbiology·Tzu-Chiao ChaoStefan Weidner
May 1, 1980·Applied and Environmental Microbiology·J OlivaresE J Bedmar
Aug 19, 2006·Journal of Bacteriology·Ana Domínguez-FerrerasJuan Sanjuán
Feb 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jun-ya KatoSueharu Horinouchi
Dec 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David M Byers, Huansheng Gong

❮ Previous
Next ❯

Citations

Aug 27, 2009·Applied Microbiology and Biotechnology·Domingo MiranzoFrancisco Malpartida
Oct 3, 2009·Microbiology·Yadira Dávila-MartínezIsabel M López-Lara
Sep 25, 2012·FEMS Microbiology Reviews·Andreas F HaagGail P Ferguson
Nov 28, 2017·Frontiers in Microbiology·Jin-Cheng MaHai-Hong Wang
Oct 9, 2018·Frontiers in Microbiology·Wei ChenChristopher Davies
Jun 3, 2018·Applied Microbiology and Biotechnology·Aaron M Marcella, Adam W Barb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.