PMID: 11926249Apr 3, 2002Paper

Smokeless tobacco extracts modulate keratinocyte and fibroblast growth in organotypic culture

Journal of Dental Research
Y WangJ A Garlick

Abstract

Smokeless tobacco is associated with pathologic alterations of the oral mucosa, yet its direct effects on human keratinocytes and fibroblasts in stratified squamous epithelium are not well-understood. We hypothesized that smokeless tobacco could modulate the growth of keratinocytes and fibroblasts in an in vivo-like, organotypic tissue model. To test this, we exposed organotypic cultures for 3 days to smokeless tobacco aqueous extracts and determined the changes in morphology and proliferation of human keratinocytes and fibroblasts. All smokeless tobaccos stimulated keratinocyte proliferation at low doses (0.25% w/v) and suppressed growth at higher doses (> 0.5% w/v). In contrast, smokeless tobacco extracts promoted fibroblast growth at all concentrations without inducing fibroblast turnover. Fibroblasts and keratinocytes, therefore, were differentially affected by smokeless tobacco extracts in an organotypic tissue model, suggesting incipient changes that may occur in vivo.

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Citations

Dec 10, 2003·Oral Oncology·Marina MerneStina Syrjänen
Dec 5, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Michael D JohnsonPeter G Shields
Jul 23, 2008·Molecular Cancer Research : MCR·Jean-Philippe CoppeAna Krtolica
Mar 27, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mitchell KlausnerJoseph Kubilus
Jan 24, 2007·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·K A A S Warnakulasuriya, R Ralhan
Oct 7, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Daniela Elena CosteaAnne Christine Johannessen
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Jan 2, 2010·Journal of Dental Research·O M LukanduD E Costea
Jun 1, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carl G SimonFrancis W Wang

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