Smokeless tobacco impacts oral microbiota in a Syrian Golden hamster cheek pouch carcinogenesis model

Anaerobe
Jinshan JinHuizhong Chen

Abstract

The use of smokeless tobacco products (STPs) can cause many serious health problems. The oral microbiota plays important roles in oral and systemic health, and the disruption in the oral microbial population is linked to periodontal disease and other health problems. To assess the impact of smokeless tobacco on oral microbiota in vivo, high-throughput sequencing was used to examine the oral microbiota present in Syrian Golden hamster cheek pouches. Sixteen hamsters were divided into four groups and treated with the STP Grizzly snuff (0, 2.5, 25, or 250 mg) twice daily for 4 weeks. After 0, 1, 2, 3, and 4 weeks of treatment, bacterial genomic DNA was extracted from oral swabs sampled from the cheek pouches of the hamsters. The oral bacterial communities present in different hamster groups were characterized by sequencing the hypervariable regions V1-V2 and V4 of 16S rRNA using the Illumina MiSeq platform. Fifteen phyla, 27 classes, 59 orders, 123 families, and 250 genera were identified from 4,962,673 sequence reads from the cheek pouch samples. The bacterial diversity and taxonomic abundances for the different treatment groups were compared to the non-treated hamsters. Bacterial diversity was significantly decreased after 4 wee...Continue Reading

References

Mar 1, 1992·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·D J SummerlinR Potter
Jan 1, 1980·The Journal of the American Dental Association·R E GoingL D Haugh
Oct 15, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P Grasso, A H Mann
Mar 30, 2002·Journal of Clinical Microbiology·Kathryn L Ruoff
Apr 24, 2003·Journal of Dental Research·P S KumarE J Leys
Oct 26, 2007·Central European Journal of Public Health·Sajith VellappallyPilathadka Shriharsha
Dec 17, 2009·BMC Microbiology·Egija ZauraWim Crielaard
Nov 3, 2012·Tobacco Control·Cristine D DelnevoPamela M Ling
Dec 11, 2013·Periodontology 2000·Robert J Palmer
May 24, 2014·The Cancer Journal·Dominique S Michaud, Jacques Izard
Dec 3, 2014·Immunology Letters·Massimo Costalonga, Mark C Herzberg
Dec 17, 2014·Periodontology 2000·Andrea Monti-HughesMaria E Itoiz
Nov 20, 2016·British Dental Journal·M KilianE Zaura
Dec 31, 2016·Journal of Clinical Periodontology·Lewis WinningGerard J Linden
Apr 22, 2017·Periodontology 2000·Samuel ChanMalcolm J West

❮ Previous
Next ❯

Citations

Jul 10, 2020·BMC Microbiology·Esam HalboubNezar Noor Al-Hebshi
Nov 24, 2020·Periodontology 2000·Nurcan Buduneli
Jan 15, 2021·Scientific Reports·Divya GopinathNewell W Johnson
Jun 7, 2021·Anaerobe·Mohammad SajidMausumi Bharadwaj
Jun 11, 2021·Applied Microbiology and Biotechnology·A J Rivera, R E Tyx
Aug 27, 2021·Clinical Oral Investigations·Divya GopinathNewell W Johnson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actinomycosis

Actinomycosis is a rare, chronic, and slowly progressive granulomatous disease caused by filamentous Gram positive anaerobic bacteria from the Actinomycetaceae family (genus Actinomyces). The disease is characterised by the formation of painful abscesses in the mouth, lungs,breast or gastrointestinal tract. Discover the latest research on actinomycosis here.

© 2022 Meta ULC. All rights reserved