Smoking is associated with increased telomerase activity in short-term cultures of human bronchial epithelial cells

Cancer Letters
Hyeon-Woo YimJack A Taylor

Abstract

Telomerase plays an important role in the maintenance of telomere ends in normal and tumor cells and ectopic expression can immortalize human bronchial epithelial (HBE) cells. We assessed telomerase activation, growth properties and methylation status in the hTERT promoter in a panel of HBE cell cultures in relation to smoking and previous lung cancer history. HBE cells were obtained from a total of 26 subjects, six of whom were lifelong non-smokers, while 20 subjects had a smoking history, including seven who had lung carcinoma. Telomerase activity was determined using the telomeric repeat amplification protocol (TRAP). Maximum passage number and time to senescence were also determined through extended culturing. The distribution of the telomerase activity between ever-smokers and never-smokers was significantly different (P=0.03, F-test), and there was a strong correlation between telomerase activity and the number of pack-years smoked (P=0.0012, F-test for slope). A small difference in telomerase activity was observed according to lung cancer status (P=0.02, F-test). Telomerase activity was not correlated with maximum passage number after extended culturing or with time to senescence. None of the HBE cultures demonstrated me...Continue Reading

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Citations

Jan 26, 2013·Carcinogenesis·Ariela L Marshall, David C Christiani
Nov 13, 2008·Risk Analysis : an Official Publication of the Society for Risk Analysis·Louis Anthony Cox
Feb 25, 2009·Cellular & Molecular Biology Letters·Mojca StrazisarDamjan Glavac
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