Smoking specifically induces metallothionein-2 isoform in human placenta at term

Toxicology
Ana Maria RoncoMiguel N Llanos

Abstract

Recently, we reported the presence of higher levels of metallothionein (MT) in placentas of smokers compared to non-smokers. In the present study, we designed experiments to separate and evaluate two isoforms of MT (MT-1 and MT-2) in placentas of smokers and non-smokers. Metallothionein was extracted and separated by ion-exchange high performance liquid chromatography (HPLC), previous saturation with cadmium chloride. Two peaks eluting at 6 and 12.5 min, corresponding to MT-1 and MT-2, respectively, were obtained. Metallothionein present in both peaks was identified by Western blot analysis using a monoclonal antibody directed against MT-1 and MT-2. Each isoform concentration was calculated after measuring its cadmium content by atomic absorption spectrometry with inductively coupled-plasma. In placentas of smokers, MT-2 levels increased by seven-fold compared to non-smokers, whereas MT-1 was not changed. Total placental cadmium and zinc concentrations, determined by atomic absorption spectrometry and neutron activation analysis, respectively, were higher in smokers. Metallothioneins levels were clearly in excess to bind all cadmium ions present in placentas. However, most of placental zinc remains unbound to MTs, although as m...Continue Reading

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Citations

Jul 16, 2009·PloS One·Ole Haagen NielsenJørgen Olsen
May 18, 2012·Environmental Health Perspectives·María D Esteban-VasalloBeatriz Perez-Gomez
Nov 20, 2015·Metallomics : Integrated Biometal Science·Gordon W IrvineMartin J Stillman
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Jan 13, 2015·Journal of Environmental and Public Health·Anders C Erickson, Laura Arbour
Nov 13, 2012·Environmental Research·E AmayaN Olea
Aug 2, 2011·American Journal of Obstetrics and Gynecology·Elena SbranaKjersti Aagaard-Tillery
May 4, 2017·Metallomics : Integrated Biometal Science·Judith S SchellerMartin J Stillman
Aug 2, 2012·Nutrients·Carmen Marino Donangelo, Janet C King

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