Smooth muscle adaptation and recovery of contractility after massive small bowel resection in rats

Experimental Biology and Medicine
Jie ChenWei Cai

Abstract

Previous studies have suggested that massive small bowel resection (mSBR) compromises the normal intestinal processes of digestion and absorption, and requires an adaptive response to regain full function and reinstate coordinated contractile activity of the circular smooth muscle. This study was designed to investigate spontaneous contractile activity of circular smooth muscle using the mSBR rat model and to determine the functional role of M(2) and M(3) muscarinic acetylcholine receptors (mAChR) in this process. Male Sprague-Dawley rats underwent an 80% proximal SBR or sham operation. Markers of adaptation, including villus and microvillus height, were analyzed by hematoxylin and eosin staining and transmission electron microscopy. Contractility was measured by attaching the distal ileum strips to strain gauge transducers and exposing the tissue to varying doses of the cholinergic agonist carbachol. Protein expressions of M(2)- and M(3)-mAChR in intestinal smooth muscle (ISM) were detected by Western blot. Following mSBR, the ISM showed perturbed spontaneous rhythmic contraction, irregular amplitude and slow frequency by muscle strip test. However, by two weeks after mSBR, the contractile function of circular smooth muscle wa...Continue Reading

References

Nov 1, 1995·Journal of Pediatric Gastroenterology and Nutrition·R B ScottD T Tan
Feb 15, 1996·The Journal of Surgical Research·B L NguyenE M Quigley
May 1, 2001·Seminars in Pediatric Surgery·D L Sigalet
Mar 11, 2003·Annual Review of Nutrition·Thomas R ZieglerDean P Jones
Apr 3, 2003·Gastroenterology·Alan L BuchmanJon Fryer
Feb 24, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Toshimitsu Uchiyama, Russell Chess-Williams
Dec 24, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K-J WonH Ozaki
Jun 15, 2006·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K KinoshitaH Ozaki
Aug 22, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael A HelmrathSusan J Henning
Jan 16, 2007·Digestive Diseases and Sciences·Jan Henrik StorkholmHans Gregersen
Jun 30, 2007·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Takashi OhamaHiroshi Ozaki
Jun 19, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J ChenB A Perrino
Jun 19, 2008·Journal of Pediatric Surgery·Colin A MartinBrad W Warner
Jun 19, 2008·Journal of Pediatric Surgery·Janice A TaylorBrad W Warner
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Cindy C TaiSaverio Bellusci

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Citations

Jun 27, 2013·World Journal of Gastroenterology : WJG·Jie ChenWei Cai
Sep 24, 2015·Iranian Journal of Pediatrics·Jie ChenWei Cai
Mar 29, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yi CaoWei Cai

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Methods Mentioned

BETA
light microscopy
electrophoresis

Software Mentioned

SigmaStat
SMUP
SPSS

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