Smooth muscle alpha-actin expression in rat hepatic stellate cell is regulated by nitric oxide and cGMP production

Biochemical and Biophysical Research Communications
N KawadaK Kobayashi

Abstract

Mechanism by which lipopolysaccharide (LPS) and interferon gamma (IFN gamma) downregulate smooth muscle (SM) alpha-actin expression in rat hepatic stellate cell was studied. Culture-induced SM alpha-actin expression was suppressed in the presence of LPS (> 5 ng/ml) and/or IFN gamma (> 1 U/ml) dose-dependently. Inhibition of nitric oxide (NO) production by N omega-nitro-L-arginine (LNA) canceled such an inhibitory effect of LPS and/or IFN gamma. LPS and/or IFN gamma increased cellular level of cGMP in an LNA inhibitable manner, and cGMP analogues attenuated SM alpha-actin expression of the cells. These results indicate that NO-dependent increase of cellular cGMP level mediates the inhibitory effect of LPS and IFN gamma on SM alpha-actin expression in stellate cells.

Citations

Jan 29, 2010·European Journal of Orthodontics·Richard G JansenJohannes W Von den Hoff
Jul 15, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Zhixia DongJianhua Wang
Sep 30, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christa BoerPieter Sipkema
Apr 24, 2010·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Jun AnNaohisa Ishikawa
Jul 23, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jonathan A FallowfieldJohn P Iredale
Sep 1, 1998·Journal of Gastroenterology and Hepatology·Norifumi KawadaMasayasu Inoue

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