Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle

Journal of Cell Science
Samudra S GangopadhyayKathleen G Morgan

Abstract

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility remain unclear. Calponin (CaP), an actin-binding protein and PKC substrate, mediates signaling through ERK1/2. We report here that CaP sequences containing the CaP homology (CH) domain bind to the C-terminal 251 amino acids of smooth-muscle archvillin (SmAV), a new splice variant of supervillin, which is a known actin- and myosin-II-binding protein. The CaP-SmAV interaction is demonstrated by reciprocal yeast two-hybrid and blot-overlay assays and by colocalization in COS-7 cells. In differentiated smooth muscle, endogenous SmAV and CaP co-fractionate and co-translocate to the cell cortex after stimulation by agonist. Antisense knockdown of SmAV in tissue inhibits both the activation of ERK1/2 and contractions stimulated by either agonist or PKC activation. This ERK1/2 signaling and contractile defect is similar to that observed in CaP knockdown experiments. In A7r5 smooth-muscle cells, PKC activation by phorbol esters induces the reorganization of endogenous, membrane-localized SmAV and microfilament-associated CaP into podosome-like structures that also contain F-actin, nonmuscle myosin IIB and...Continue Reading

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Citations

May 2, 2009·The Journal of Biological Chemistry·Samudra S GangopadhyayKathleen G Morgan
Dec 26, 2008·Molecular Biology of the Cell·Jessica L CrowleyElizabeth J Luna
Jan 4, 2007·Genome Research·Sara J CooperRichard M Myers
Nov 20, 2012·Journal of Neuroinflammation·Tamjeed A SiddiquiLyanne C Schlichter
Sep 17, 2002·The Japanese Journal of Physiology·Shinsuke NakayamaJoseph F Clark
Feb 6, 2013·The Journal of Biological Chemistry·Zhiyou Fang, Elizabeth J Luna
Aug 2, 2006·The Journal of Cell Biology·Norio TakizawaElizabeth J Luna
Aug 18, 2009·Journal of Molecular Biology·Jeffrey W BrownC James McKnight
May 29, 2008·Journal of Cellular and Molecular Medicine·Susanne Vetterkind, Kathleen G Morgan
Jan 6, 2009·Journal of Cellular and Molecular Medicine·H R KimK G Morgan
Dec 22, 2006·Cell Motility and the Cytoskeleton·Melissa A SenetarRichard O McCann
Mar 13, 2009·Journal of Cellular and Molecular Medicine·Christian W Kreipke, Jose A Rafols
Jun 12, 2013·Cytoskeleton·Shalini NagLeslie D Burtnick
Mar 24, 2010·Cytoskeleton·Tara C SmithElizabeth J Luna
Feb 7, 2015·Cytoskeleton·Kyonghee SonElizabeth J Luna
Jan 22, 2015·Human Molecular Genetics·Janelle M SpinazzolaElisabeth R Barton
Aug 27, 2014·BioMed Research International·Cornelia TolgEva A Turley
Jun 16, 2016·Frontiers in Cell and Developmental Biology·Berta Casar, Piero Crespo
Sep 28, 2013·Biochemistry·Stanislav O FedechkinSerge L Smirnov
Nov 9, 2016·Biochemical and Biophysical Research Communications·Xueran ChenZhiyou Fang
Dec 19, 2009·American Journal of Physiology. Cell Physiology·Hak Rim KimKathleen G Morgan
Aug 12, 2020·Brain : a Journal of Neurology·Carola Hedberg-OldforsCordula Knopp
Mar 15, 2008·The Biochemical Journal·Samudra S GangopadhyayKathleen G Morgan
Oct 11, 2007·Journal of Cell Science·Norio TakizawaElizabeth J Luna
Jul 28, 2005·Journal of Cell Science·Cynthia GallantKathleen G Morgan
Feb 22, 2012·Journal of Cell Science·Ridhirama BhuwaniaStefan Linder

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