Smooth muscle-like Ca2+ -regulation of actin-myosin interaction in adult jellyfish striated muscle

Scientific Reports
Hiroyuki TanakaKeisuke Ohashi

Abstract

Cnidaria is an animal phylum, whose members probably have the most ancestral musculature. We prepared and characterized, for the first time to our knowledge, native actomyosin from the striated myoepithelium of the adult moon jelly Aurelia sp. The actomyosin contained myosin, paramyosin-like protein, Ser/Thr-kinase, actin, and two isoforms of tropomyosin, but not troponin, which is known to activate contraction dependent on intracellular Ca2+ signaling in almost all striated muscles of bilaterians. Notably, the myosin comprised striated muscle-type heavy chain and smooth muscle-type regulatory light chains. In the presence of Ca2+, the Mg-ATPase activity of actomyosin was stimulated and Ser21 of the regulatory light chain was concomitantly phosphorylated by the addition of calmodulin and myosin light chain kinase prepared from chicken smooth muscle. Collectively, these results suggest that, similar to smooth muscle, the contraction of jellyfish striated muscle is regulated by Ca2+-dependent phosphorylation of the myosin light chain.

References

May 5, 1988·Nature·Y MatsumotoM Aikawa
Dec 14, 1970·Journal of Molecular Biology·J Kendrick-JonesA G Szent-Györgyi
Jan 1, 1980·Annual Review of Biochemistry·R S Adelstein, E Eisenberg
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H V Goodson, J A Spudich
Jan 1, 1993·Cell Motility and the Cytoskeleton·R E CheneyM S Mooseker
Apr 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K Maruyama
Feb 1, 1997·Journal of Muscle Research and Cell Motility·P J GallagherJ T Stull
Jul 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J M Squire, E P Morris
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 7, 2005·Developmental Biology·Katja Seipel, Volker Schmid
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma
Jan 16, 2007·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Patrick Michael Burton
Dec 7, 2007·Biochemical and Biophysical Research Communications·Akio NakamuraKazuhiro Kohama
Apr 1, 2011·The Journal of Comparative Neurology·Stefano PirainoHeinrich Reichert
Nov 23, 2011·Journal of Muscle Research and Cell Motility·Belinda Bullard, Annalisa Pastore
Jul 6, 2012·Nature·Patrick R H SteinmetzUlrich Technau
Jan 21, 2014·Current Biology : CB·Björn FuchsKonstantin Khalturin
Jun 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael J AbramsLea Goentoro
Oct 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Guidenn SulbaránRoger Craig
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Feb 9, 2017·Frontiers in Cell and Developmental Biology·Lucas Leclère, Eric Röttinger

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Win
BLAST
Genetyx
Excel
Mascot
MEGA7
TrEMBL

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.