Smooth Muscle Precursor Cells Derived from Human Pluripotent Stem Cells for Treatment of Stress Urinary Incontinence

Stem Cells and Development
Zhe WangBertha Chen

Abstract

There is great interest in using stem cells (SC) to regenerate a deficient urethral sphincter in patients with urinary incontinence. The smooth muscle component of the sphincter is a significant contributor to sphincter function. However, current translational efforts for sphincter muscle restoration focus only on skeletal muscle regeneration because they rely on adult mesenchymal SC as cell source. These adult SC do not yield sufficient smooth muscle cells (SMCs) for transplantation. We may be able to overcome this limitation by using pluripotent stem cell (PSC) to derive SMCs. Hence, we sought to investigate whether smooth muscle precursor cells (pSMCs) derived from human PSCs can restore urethral function in an animal model generated by surgical urethrolysis and ovariectomy. Rats were divided into four groups: control (no intervention), sham saline (surgery + saline injection), bladder SMC (surgery + human bladder SMC injection), and treatment (surgery + pSMC injection, which includes human embryonic stem cell (hESC) H9-derived pSMC, episomal reprogrammed induced pluripotent stem cells (iPSCs)-derived pSMC, or viral reprogrammed iPSC-derived pSMC). pSMCs (2 × 10(6) cells/rat) were injected periurethrally 3 weeks postsurgery....Continue Reading

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Dec 3, 2014·Advanced Drug Delivery Reviews·Longkun LiYuanyuan Zhang
Sep 16, 2017·Pediatric Research·Philip M IannacconeArun K Sharma
Nov 15, 2019·Cell Transplantation·Aline Yen Ling Wang, Charles Yuen Yung Loh
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Jun 6, 2020·Biomedical Materials·Igor VasyutinYuanyuan Zhang

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Methods Mentioned

BETA
biopsies
dissection
FACS
bioluminescence imaging
biopsy

Software Mentioned

AcqKnowledge
JMP pro

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