Smooth muscle-selective alternatively spliced exon generates functional variation in Cav1.2 calcium channels

The Journal of Biological Chemistry
Ping LiaoTuck Wah Soong

Abstract

Voltage-gated calcium channels play a major role in many important processes including muscle contraction, neurotransmission, excitation-transcription coupling, and hormone secretion. To date, 10 calcium channel alpha(1)-subunits have been reported, of which four code for L-type calcium channels. In our previous work, we uncovered by transcript-scanning the presence of 19 alternatively spliced exons in the L-type Ca(v)1.2 alpha(1)-subunit. Here, we report the smooth muscle-selective expression of alternatively spliced exon 9(*) in Ca(v)1.2 channels found on arterial smooth muscle. Specific polyclonal antibody against exon 9(*) localized the intense expression of 9(*)-containing Ca(v)1.2 channels on the smooth muscle wall of arteries, but the expression on cardiac muscle was low. Whole-cell patch clamp recordings of the 9(*)-containing Ca(v)1.2 channels in HEK293 cells demonstrated -9 and -11-mV hyperpolarized shift in voltage-dependent activation and current-voltage relationships, respectively. The steady-state inactivation property and sensitivity to blockade by nifedipine of the +/-exon 9(*) splice variants were, however, not significantly different. Such cell-selective expression of an alternatively spliced exon strongly ind...Continue Reading

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Citations

Jan 20, 2009·Pflügers Archiv : European journal of physiology·Ping LiaoTuck Wah Soong
Mar 6, 2009·Pflügers Archiv : European journal of physiology·Ping LiaoTuck Wah Soong
Nov 17, 2009·Pflügers Archiv : European journal of physiology·Ping Liao, Tuck Wah Soong
Jul 1, 2009·Molecular and Cellular Biology·Zhen Zhi TangDouglas L Black
Jun 9, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Xiaoyang ChengJonathan H Jaggar
Sep 1, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Matthew A NystoriakGeorge C Wellman
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Oct 4, 2015·Pflügers Archiv : European journal of physiology·Paul FransenGilles W De Keulenaer
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Oct 31, 2019·Pflügers Archiv : European journal of physiology·Bo ChenPing Liao
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Jan 1, 2012·ISRN Molecular Biology·Nikolai M Soldatov
Jan 1, 2013·ISRN Biochemistry·Nikolai M Soldatov
Feb 20, 2015·The Journal of Biological Chemistry·Ping LiaoTuck Wah Soong
Oct 23, 2020·Circulation Research·Arianne PapaSteven O Marx

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