SmpB functions in various steps of trans-translation

Nucleic Acids Research
Kyoko Hanawa-SuetsuguAkira Muto

Abstract

tmRNA has a dual function as a tRNA and an mRNA to facilitate trans-translation, in which a ribosome can switch between translation of a truncated mRNA and the tmRNA's tag sequence. SmpB is a tmRNA binding protein that has been identified to be essential for trans-translation in vivo. To further study the function of SmpB, an S30 fraction from an Escherichia coli strain, in which the set of genes for SmpB and tmRNA has been deleted from the genome, and His-tagged SmpB active in trans-translation were prepared. The SmpB-depleted S30 fraction had an ability to facilitate poly(U)-dependent tag-peptide synthesis in vitro when purified His-tagged SmpB was exogenously added together with tmRNA, although SmpB was not required for in vitro poly(U)-dependent poly(Phe) synthesis. It was also found that depletion of SmpB leads to a decrease in the level of tmRNA in the cell. In addition, SmpB considerably enhanced the aminoacylation of tmRNA by alanyl-tRNA synthetase in vitro. The aminoacylation enhancement by SmpB, the binding of SmpB to tmRNA and the effect of depletion of SmpB on the expression level of tmRNA in the cell were all affected by some mutations in the tRNA-like domain which cause a defect in ribosome binding leading to a tr...Continue Reading

References

Jul 1, 1991·Journal of Molecular Recognition : JMR·K TamuraM Shimizu
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y KomineH Inokuchi
Aug 15, 1994·Analytical Biochemistry·H TsuruiT Shirai
Mar 1, 1996·Journal of Biochemistry·Y KomineH Inokuchi
Dec 16, 1996·FEBS Letters·T TadakiA Muto
Jan 1, 1996·Biochimie·B FeldenR F Gesteland
May 23, 1997·Journal of Molecular Biology·H HimenoA Muto
Mar 28, 1998·Trends in Biochemical Sciences·A MutoH Himeno
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z LiM P Deutscher
Aug 17, 1999·The EMBO Journal·E D Roche, R T Sauer
Oct 3, 1999·The EMBO Journal·K P WilliamsD P Bartel
Dec 11, 1999·Nucleic Acids Research·K P Williams
Dec 11, 1999·Nucleic Acids Research·C Zwieb, J Wower
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A MutoH Himeno
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A W Karzai, R T Sauer

❮ Previous
Next ❯

Citations

Nov 1, 1979·Biulleten' eksperimental'noĭ biologii i meditsiny·G M Elbakidze, G Sh Vashakmadzde
Jun 22, 2005·Journal of Molecular Biology·Natalia IvanovaMåns Ehrenberg
Apr 23, 2004·Current Opinion in Structural Biology·Peter W HaebelNenad Ban
Aug 10, 2007·Nature Reviews. Cancer·Kristopher K Frese, David A Tuveson
Jul 26, 2002·Molecular Microbiology·Justine CollierPhilippe Bouloc
Jan 25, 2003·European Journal of Biochemistry·Victor G Stepanov, Jens Nyborg
Dec 21, 2006·The Journal of Biological Chemistry·Reynald GilletJoachim Frank
Apr 5, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yang SongBrian J Wilkinson
Aug 6, 2004·Nucleic Acids Research·Takayuki KonnoHyouta Himeno
Apr 30, 2005·Nucleic Acids Research·Laurent MetzingerBrice Felden
Jun 24, 2005·Nucleic Acids Research·Natalia IvanovaLovisa Holmberg Schiavone
Oct 6, 2005·Nucleic Acids Research·Krisana AsanoHyouta Himeno
Oct 26, 2007·Nucleic Acids Research·Daisuke KuritaHyouta Himeno
Jan 4, 2003·Journal of Bacteriology·Kenneth C Keiler, Lucy Shapiro
Feb 13, 2007·Annual Review of Biochemistry·Sean D Moore, Robert T Sauer
Jun 19, 2008·Annual Review of Microbiology·Kenneth C Keiler
Oct 29, 2009·RNA·Elizaveta Y BugaevaOlga A Dontsova
Apr 3, 2007·RNA·Natalia IvanovaMåns Ehrenberg
Mar 24, 2004·RNA·Yannick JacobB Franz Lang
Nov 27, 2004·Bioscience, Biotechnology, and Biochemistry·Takahiro OkadaMakoto Kimura
Feb 2, 2006·PLoS Pathogens·Nihal A OkanA Wali Karzai
Jan 22, 2011·Journal of Nucleic Acids·Daisuke KuritaHyouta Himeno
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Thomas R SundermeierA Wali Karzai
May 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yoshitaka BesshoShigeyuki Yokoyama
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sukhjit KaurJoachim Frank
Apr 9, 2008·FEBS Letters·Elizaveta Y BugaevaOlga A Dontsova
Feb 1, 2003·FEBS Letters·Tatsuhiko SomeyaGota Kawai
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson, Knud H Nierhaus
Sep 17, 2005·Biochimie·Matthieu SaguyBrice Felden
Oct 23, 2013·Tuberculosis·Yoann Personne, Tanya Parish
Jun 15, 2011·Biochimie·Kenneth C Keiler, Nitya S Ramadoss
Dec 3, 2014·Biochimie·Hyouta HimenoTatsuhiko Abo
Mar 23, 2007·Journal of Molecular Biology·Hao QiTakuya Ueda
Apr 30, 2014·Frontiers in Genetics·Hyouta HimenoAkira Muto
Sep 18, 2014·Frontiers in Microbiology·Mickey R Miller, Allen R Buskirk
Nov 29, 2016·Scientific Reports·Romain Brunel, Xavier Charpentier
Apr 8, 2004·The Journal of Biological Chemistry·Marc HallierBrice Felden
Dec 15, 2004·The Journal of Biological Chemistry·Yannick JacobKelly P Williams

❮ Previous
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