SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis

PloS One
Xiangli YeYuequn Wang

Abstract

Previous studies have demonstrated that Smyd1 plays a critical role in cardiomyocyte differentiation, cardiac morphogenesis and myofibril organization. In this study, we uncovered a novel function of Smyd1 in the regulation of endothelial cells (ECs). Our data showed that Smyd1 is expressed in vascular endothelial cells, and knockdown of SMYD1 in endothelial cells impairs EC migration and tube formation. Furthermore, Co-IP and GST pull-down assays demonstrated that SMYD1 is associated with the Serum Response Factor (SRF). EMSA assays further showed that SMYD1 forms a complex with SRF and enhances SRF DNA binding activity. Our studies indicate that SMYD1 serves as an SRF-interacting protein, enhances SRF DNA binding activity, and is required for EC migration and tube formation to regulate angiogenesis.

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Citations

Jun 30, 2018·European Journal of Haematology·Vered YahalomElon Pras
Jul 7, 2019·Pediatric Cardiology·Garrett N CoyanMahesh S Sharma
Feb 13, 2018·Current Opinion in Physiology·Christopher TracySarah Franklin

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Methods Mentioned

BETA
pull-down
PCR
co-immunoprecipitation
Co-IP
electrophoretic mobility shift
electrophoresis
scraping

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