Snail controls the mesenchymal phenotype and drives erlotinib resistance in oral epithelial and head and neck squamous cell carcinoma cells.

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
Miranda DennisMaie A St John

Abstract

The presence of regional metastases in patients with head and neck squamous cell carcinoma (HNSCC) is a common and adverse event associated with poor prognosis. The authors' recent work on human HNSCC tissues underlies Snail's role as a molecular prognostic marker for HNSCC. Snail positivity is significantly predictive of poorly differentiated, lymphovascular invasive, and regionally metastatic tumors. Here, the authors investigate the capacity of Snail to drive epithelial-mesenchymal transition (EMT) in human oral epithelial cell lines and its ability to confer drug resistance. Snail was overexpressed in HNSCC and oral epithelial cell lines. Anchorage independent growth assays, wound healing assays, invasion and migration assays, spheroid modeling, and cell survival assays were performed. Academic tertiary medical center. Snail overexpressing HNSCC (OSC, Tu212, Tu686) and oral epithelial cell lines (HOK 16-B, OKF-6) were evaluated using assays for wound healing, invasion and migration, 3-dimensional growth, Western blot, and immunofluorescence. The overexpression of Snail in human HNSCC and oral epithelial cell lines drives EMT. The transfection of Snail confers the expression of a mesenchymal molecular signature, including do...Continue Reading

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Citations

Aug 7, 2012·Seminars in Cancer Biology·Philip L LeopoldHongjun Wang
Jan 16, 2014·Epidemiology and Infection·E M AbdelwhabT C Mettenleiter
May 12, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Zihang LingXiaoan Tao
Oct 18, 2019·Journal of Clinical Medicine·Ilya YakavetsLina Bezdetnaya
Apr 4, 2020·Molecular Metabolism·Aarif Siddiqui, Paolo Ceppi
Dec 18, 2020·International Journal of Molecular Sciences·Ravi Teja Chitturi SuryaprakashCamile S Farah
Jul 3, 2021·Cancers·Rogelio González-GonzálezRonell Bologna-Molina

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Methods Mentioned

BETA
electrophoresis
transfection

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