Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles

Journal of Cell Science
Michela RigoniOrnella Rossetto

Abstract

The mechanisms of action of four snake presynaptic phospholipase A2 neurotoxins were investigated in cultured neurons isolated from various parts of the rat brain. Strikingly, physiological concentrations of notexin, beta-bungarotoxin, taipoxin or textilotoxin induced a dose-dependent formation of discrete bulges at various sites of neuronal projections. Neuronal bulging was paralleled by the redistribution of the two synaptic vesicle markers synaptophysin I (SypI) and vesicle-attached membrane protein 2 (VAMP2) to the bulges, and by the exposure of the luminal domain of synaptotagmin on the cell surface. These neurotoxins induced glutamate release from cultured neurons similarly to the known evoked release of acetylcholine from neuromuscular junctions. In addition, partial fragmentation of F-actin and neurofilaments was observed in neurons, but not in astrocytes. These findings indicate that these snake presynaptic neurotoxins act with by same mechanism and that the observed phenotype results from the fusion of synaptic vesicles with the plasma membrane not balanced by an adequate membrane retrieval. These changes closely resemble those occurring at neuromuscular junctions of intoxicated animals and fully qualify these primary...Continue Reading

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Citations

Sep 24, 2008·The Journal of Biological Chemistry·Michela RigoniCesare Montecucco
Oct 17, 2013·PLoS Neglected Tropical Diseases·Udaya K RanawakaH Janaka de Silva
Feb 1, 2014·Brain Research·Nathalia Delazeri de CarvalhoMaria Regina Lopes Sandoval
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Jul 11, 2014·Toxicon : Official Journal of the International Society on Toxinology·Jernej ŠribarIgor Križaj
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Apr 4, 2009·Toxicon : Official Journal of the International Society on Toxinology·Erik TedescoCesare Montecucco

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