Snake venom characteristic peptides: novel fingerprints for species identification by sheathless capillary electrophoresis-electrospray ionization-mass spectrometry

The Analyst
Ying LiuXin-Xiang Zhang

Abstract

Snake venom is a complex mixture mainly consisting of proteins and peptides which varies with different species. These variations lead to different toxic mechanisms and different anti-venom serums for treatment and the determination of their use as drugs. Hence, it is important to develop a sensitive and reliable method to identify the species of snakes from venoms. Herein, we present a novel strategy based on the sheathless capillary electrophoresis-electrospray ionization-mass spectrometry (CESI-MS) system to characterize snake venom proteins. Through the determination of peptides, we found the characteristic peptides of 8 different snakes with high sensitivity (1 μg mL-1) and high selectivity, which provided a reliable method for the species identification and purity detection of snake venom samples.

References

Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Neville Marsh, Vaughan Williams
Nov 17, 2009·Journal of Microbiological Methods·Ali R VaezzadehDenis F Hochstrasser
Mar 31, 2011·Toxicon : Official Journal of the International Society on Toxinology·Cho Yeow Koh, R Manjunatha Kini
Feb 2, 2013·Journal of Proteome Research·Linda SwitzarWilfried M A Niessen
Apr 18, 2013·Asian Pacific Journal of Tropical Biomedicine·Vivek Kumar VyasUtsav Parmar
Jun 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicholas R CasewellRobert A Harrison
Nov 22, 2016·Toxicon : Official Journal of the International Society on Toxinology·Yujing HuJi-Fu Wei
May 4, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Bruno Lomonte, Juan J Calvete
Nov 18, 2018·Toxins·Aisha MunawarChristian Betzel

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Methods Mentioned

BETA
electrophoresis
ELISA

Software Mentioned

BLAST

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