Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins

Toxicon : Official Journal of the International Society on Toxinology
Andreas H LaustsenBruno Lomonte

Abstract

The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5%; including 24.3% cytotoxins and 53.2% neurotoxins) and phospholipases A2 (13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, l-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most relevant lethal components, categorized by means of a 'toxicity score', were α-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical α-neurotoxins were obtained. As expected, no neutralization potential against N. kao...Continue Reading

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Citations

Jul 15, 2015·Toxicon : Official Journal of the International Society on Toxinology·Andreas H LaustsenBruno Lomonte
Mar 15, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Kae Yi TanNget Hong Tan
Aug 5, 2015·Toxicon : Official Journal of the International Society on Toxinology·Andreas H LaustsenJosé María Gutiérrez
May 11, 2016·Toxicon : Official Journal of the International Society on Toxinology·Cassandra M ModahlStephen P Mackessy
Jun 4, 2016·Toxicon : Official Journal of the International Society on Toxinology·Kitisak SintiprungratKavi Ratanabanangkoon
Oct 25, 2016·Journal of Proteomics·Line P LauridsenJosé María Gutiérrez
Oct 28, 2016·Molecular BioSystems·Vera OldratiReto Stöcklin
Dec 26, 2016·Biochimica Et Biophysica Acta. General Subjects·Johara Boldrini-FrançaEliane Candiani Arantes
May 4, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Bruno Lomonte, Juan J Calvete
Jul 30, 2019·Frontiers in Immunology·Manuela B PuccaAndreas H Laustsen
Oct 28, 2020·Toxins·Manuela B PuccaWuelton M Monteiro
Jan 29, 2021·Frontiers in Immunology·José María GutiérrezGuillermo León
Mar 21, 2020·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Cassandra M ModahlStephen P Mackessy
Apr 7, 2019·Toxicon : Official Journal of the International Society on Toxinology·Archana DekaRobin Doley
May 30, 2017·New Biotechnology·Andreas H Laustsen

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