Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila

Journal of Cell Science
Yuichi YanagihashiMikio Furuse

Abstract

Septate junctions (SJs) are the membrane specializations observed between epithelial cells in invertebrates. SJs play a crucial role in epithelial barrier function by restricting the free diffusion of solutes through the intercellular space. In arthropod species, two morphologically different types of SJs have been described: pleated septate junctions (pSJs) and smooth septate junctions (sSJs), which are specific to ectodermal and endodermal epithelia, respectively. In contrast to the recent identification of pSJ-related proteins, the molecular constituents of sSJs are mostly unknown. Here, we report the discovery of a new sSJ-specific membrane protein, designated 'Snakeskin' (Ssk). Ssk is highly concentrated in sSJs in the Drosophila midgut and Malpighian tubules. Lack of Ssk expression is embryonically lethal in Drosophila and results in defective sSJ formation accompanied by abnormal morphology of midgut epithelial cells. We also show that the barrier function of the midgut to a fluorescent tracer is impaired in ssk-knockdown larvae. These results suggest that Ssk is required for the intestinal barrier function in Drosophila.

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Citations

Oct 30, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Sima JonusaiteScott P Kelly
Mar 24, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Sima JonusaiteAndrew Donini
Sep 23, 2014·Seminars in Cell & Developmental Biology·Yasushi Izumi, Mikio Furuse
Feb 6, 2016·Journal of Cell Science·Yasushi IzumiMikio Furuse
Jan 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ruei-Jiun HungNorbert Perrimon
Jun 23, 2020·EMBO Reports·Maja C FunkMichael Boutros
Apr 9, 2013·Bioscience, Biotechnology, and Biochemistry·Chinatsu KawadaYoshihiro Nomura
Jun 27, 2012·Annals of the New York Academy of Sciences·Klaus W Beyenbach
Apr 9, 2020·American Journal of Physiology. Cell Physiology·Klaus W BeyenbachAchim Paululat
Jun 22, 2017·Annals of the New York Academy of Sciences·Mikio Furuse, Yasushi Izumi
Feb 14, 2021·Current Opinion in Insect Science·Sima Jonusaite, Aylin R Rodan
Feb 14, 2021·Biology Open·Anthony Galenza, Edan Foley
May 25, 2020·Insect Biochemistry and Molecular Biology·June-Sun YoonAngela E Douglas

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