SNAP-25, a SNARE protein, inhibits two types of K channels in esophageal smooth muscle

Gastroenterology
J JiN E Diamant

Abstract

The plasma membrane-associated soluble N-ethylmaleimide-sensitive factors attachment protein receptors (SNAREs), synaptosome-associated protein of 25 kilodaltons (SNAP-25), and syntaxin 1A, have been found to physically interact with and functionally modify membrane-spanning ion channels. Studies were performed in cat esophageal body and lower esophageal sphincter (LES) smooth muscle to (1) show the presence of SNAP-25, and (2) determine whether SNAP-25 affects K+ channel activity. Single circular muscle cells from the esophageal body and sphincter were studied. Cellular localization of SNAP-25 and K+ channel activity were assessed. SNAP-25 was found in the plasma membrane of all regions examined. Outward K+ currents in body circular muscle were mainly composed of large conductance Ca2+-activated channel currents (K(Ca), 40.1%) and delayed rectifier K+ channel currents (K(V), 54.2%). Microinjection of SNAP-25 into muscle cells caused a dose-dependent inhibition of both outward K+ currents, maximal 44% at 10(-8) mol/L. Cleavage of endogenous SNAP-25 by dialyzing botulinum neurotoxin A into the cell interior resulted in a 35% increase in outward currents. SNAP-25 protein is present in esophageal smooth muscle cells, and inhibits ...Continue Reading

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Citations

Mar 7, 2003·The Journal of Biological Chemistry·Yuk M LeungHerbert Y Gaisano
Jul 16, 2009·Reproductive Sciences·Irina D BurdThomas N Tulenko
Feb 1, 2011·The Journal of Comparative Neurology·Saori YamamoriMasami Takahashi
Sep 30, 2016·Toxins·Raffaela PeroFrancesca Lembo
Mar 28, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Arlene N JamesHenry P Parkman
Jan 24, 2009·Annals of the New York Academy of Sciences·Lori FeinshreiberIlana Lotan
Nov 26, 2010·Current Opinion in Urology·Emmanuel Chartier-KastlerFrancois Giuliano
Sep 6, 2002·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J JiH Y Gaisano
Sep 2, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vijayalakshmi GabettaKirk M McHugh
Jan 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R Ben-YoussefO Ojogho
Jan 20, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Leila NeshatianNicholas E Diamant
Apr 4, 2006·Biochemical and Biophysical Research Communications·Sunil K SaxenaBlake McConnell

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