SNAP-tag as a tool for surface immobilization

Current Pharmaceutical Design
Sinem EnginLjiljana Fruk


SNAP-tag technology has been an important tool for protein study for more than a decade and in the meanwhile has found a number of applications beyond the field of molecular biology and protein purification. Based on covalent interaction of SNAP-tag, 20 kDA mutant of DNA repair protein and benzylguanine, it enables irreversible and controllable protein modification. In this mini review, recent developments in the use of SNAP-tag for the design of protein arrays and nanoparticle biofunctionalization are presented and discussed. A short overview of other applications that paved the way to surface modifications is also given with emphasis on fluorescent labeling through the use of SNAP-tag fusion proteins. Finally, the future of the SNAP-tag methodology for surface patterning and 3D structural scaffolding is addressed.


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Related Concepts

DNA repair protein
Molecular Imaging
Covalent Interaction
Post-Translational Protein Processing
Surface Properties
Fluorescent stain
Nanocrystalline Materials
Protein Purification
Protein Arrays

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