Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Optics Express
L GaoT S Tkaczyk

Abstract

A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

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Citations

Feb 6, 2014·The Analyst·Kerstin GallerUte Neugebauer
Aug 16, 2012·Applied Physics Letters·Sergey A AlexandrovYang Liu
Jun 7, 2011·Journal of Biomedical Optics·Robert T KesterTomasz S Tkaczyk
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Jul 14, 2012·Optical Engineering : the Journal of the Society of Photo-optical Instrumentation Engineers·Nathan HagenTomasz S Tkaczyk
Sep 11, 2012·Optical Engineering : the Journal of the Society of Photo-optical Instrumentation Engineers·Noah BedardTomasz S Tkaczyk
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Sep 5, 2014·Journal of Biophotonics·Liang Gao, R Theodore Smith
Dec 15, 2017·Optical Engineering : the Journal of the Society of Photo-optical Instrumentation Engineers·Ye WangTomasz S Tkaczyk
Apr 3, 2014·Optics Letters·Ethan SchonbrunGiuseppe Di Caprio
Aug 31, 2016·International Ophthalmology Clinics·Jason G DwightTomasz S Tkaczyk
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Sep 7, 2013·Biomedical Optics Express·Giuseppe Di CaprioEthan Schonbrun
Feb 3, 2016·Neurophotonics·Soren D KoneckyBruce J Tromberg
Jul 1, 2017·Biomedical Optics Express·Jason G Dwight, Tomasz S Tkaczyk
Oct 20, 2017·Applied Optics·Oren Pe'eriMenachem Nathan
May 6, 2019·Optics Express·S Krause, T Vosch
Jun 6, 2019·Optics Express·Ye WangTomasz S Tkaczyk
Mar 4, 2020·Optics Express·Anqi LiuXiaoming Ding
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Apr 2, 2014·Optics Letters·Xing LinQionghai Dai
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Jun 6, 2019·Optics Express·Yifan WangMichael W Kudenov
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Nov 7, 2019·Optics Express·Ying FuHua Huang
Jan 19, 2012·Biomedical Optics Express·Liang GaoTomasz S Tkaczyk
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Mar 29, 2012·Optics Express·Yuri MurakamiNagaaki Ohyama
Jul 1, 2017·Biomedical Optics Express·Yu John HsuSzu-Yu Chen

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Methods Mentioned

BETA
confocal microscopy
fluorescence imaging
fluorescence microscopy

Software Mentioned

CameraLink

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