PMID: 8599762Apr 1, 1996Paper

Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex of Escherichia coli PEP carboxykinase

Nature Structural Biology
L W TariLouis T J Delbaere

Abstract

We report the 1.8 A crystal structure of adenosine triphosphate (ATP)-magnesium-oxalate bound phosphoenolpyruvate carboxykinase (PCK) from Escherichia coli. ATP binding induces a 20 degree hinge-like rotation of the N- and C-terminal domains which closes the active-site cleft. PCK possesses a novel nucleotide-binding fold, particularly in the adenine-binding region, where the formation of a cis backbone torsion angle in a loop glycine residue promotes intimate contacts between the adenine-binding loop and adenine, while stabilizing a syn conformation of the base. This complex represents a reaction intermediate analogue along the pathway of the conversion of oxaloacetate to phosphoenolpyruvate, and provides insight into the mechanistic details of the chemical reaction catalysed by this enzyme.

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Citations

Mar 17, 2004·Biochimica Et Biophysica Acta·Louis T J DelbaereHughes Goldie
May 14, 2005·The International Journal of Biochemistry & Cell Biology·Julien J H CotelesageLouis T J Delbaere
Apr 1, 1996·Nature Structural Biology·E F Pai
Dec 24, 1997·Nature Structural Biology·L W TariL T Delbaere
Jul 30, 2009·The Journal of Biological Chemistry·Gerald M Carlson, Todd Holyoak
Aug 22, 2013·Nucleic Acids Research·Kommireddy VasuValakunja Nagaraja
Oct 24, 2000·Genome Research·Y WangT Tatusova
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lei Xie, Philip E Bourne
Mar 19, 2008·The International Journal of Biochemistry & Cell Biology·Iván TobarEmilio Cardemil
May 4, 2007·The International Journal of Biochemistry & Cell Biology·Julien J H CotelesageLouis T J Delbaere
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Kengo Kinoshita, Haruki Nakamura
Nov 18, 2006·Journal of Molecular Graphics & Modelling·Chenyang ZhanYue-Lan Wang
Oct 26, 2013·Biochemical and Biophysical Research Communications·Adam D Lietzan, Martin St Maurice
Oct 29, 1998·The Journal of Biological Chemistry·E H Bursey, B K Burgess
Mar 28, 1997·The Journal of Biological Chemistry·A MatteL T Delbaere
Jan 18, 2002·The Journal of Biological Chemistry·Anne GalinierJean-Michel Jault
Jan 9, 2020·Biochimica Et Biophysica Acta. General Subjects·Akosiererem SokariboHughes Goldie
Feb 20, 2002·Journal of Molecular Biology·Pete DuntenStanley J Wertheimer
May 20, 1998·Structure·A MatteL T Delbaere
Feb 17, 2009·Structure·Akira R Kinjo, Haruki Nakamura

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