Snapshot of an oxygen intermediate in the catalytic reaction of cytochrome c oxidase

Proceedings of the National Academy of Sciences of the United States of America
Izumi IshigamiDenis L Rousseau

Abstract

Cytochrome c oxidase (CcO) reduces dioxygen to water and harnesses the chemical energy to drive proton translocation across the inner mitochondrial membrane by an unresolved mechanism. By using time-resolved serial femtosecond crystallography, we identified a key oxygen intermediate of bovine CcO. It is assigned to the PR-intermediate, which is characterized by specific redox states of the metal centers and a distinct protein conformation. The heme a3 iron atom is in a ferryl (Fe4+ = O2-) configuration, and heme a and CuB are oxidized while CuA is reduced. A Helix-X segment is poised in an open conformational state; the heme a farnesyl sidechain is H-bonded to S382, and loop-I-II adopts a distinct structure. These data offer insights into the mechanism by which the oxygen chemistry is coupled to unidirectional proton translocation.

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Citations

Mar 12, 2019·Journal of Synchrotron Radiation·Raymond G SierraSébastien Boutet
Oct 17, 2019·The FEBS Journal·Feng-Zhu ZhaoDa-Chuan Yin
Jul 14, 2020·Biotechnology and Applied Biochemistry·Linda LeoneAngela Lombardi
Apr 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Amandine MaréchalPeter R Rich
Jun 30, 2019·Analytical and Bioanalytical Chemistry·Austin EchelmeierAlexandra Ros
Sep 11, 2020·Nature Communications·Austin EchelmeierAlexandra Ros
Oct 13, 2020·Frontiers in Oncology·Jorgelindo da Veiga MoreiraMario Jolicoeur
Jan 12, 2021·Computational and Structural Biotechnology Journal·Samantha J ZieglerYannick J Bomble
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Oct 19, 2019·Journal of Molecular Biology·Giuseppe CapitanioSergio Papa
Nov 5, 2019·Biochimica Et Biophysica Acta. General Subjects·Michihiro SugaHiroshi Sugimoto
Apr 10, 2021·Journal of Applied Crystallography·Huijong HanKristina Lorenzen
Apr 18, 2021·The FEBS Journal·Harshwardhan PoddarNigel S Scrutton
May 11, 2021·Biochimica Et Biophysica Acta. Bioenergetics·T SztachovaM Fabian
May 31, 2019·Journal of the American Chemical Society·Andrew W SchaeferEdward I Solomon
Aug 28, 2021·Science·Gisela Brändén, Richard Neutze

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