Snapshot Transient Absorption Spectroscopy of Carotenoid Radical Cations in High-Light-Acclimating Thylakoid Membranes

The Journal of Physical Chemistry Letters
Soomin ParkGraham R Fleming

Abstract

Nonphotochemical quenching mechanisms regulate light harvesting in oxygenic photosynthesis. Measurement techniques for nonphotochemical quenching have typically focused on downstream effects of quenching, such as measuring reduced chlorophyll fluorescence. Here, to directly measure a species involved in quenching, we report snapshot transient absorption (TA) spectroscopy, which rapidly tracks carotenoid radical cation signals as samples acclimate to excess light. The formation of zeaxanthin radical cations, which is possible evidence of zeaxanthin-chlorophyll charge-transfer (CT) quenching, was investigated in spinach thylakoids. Together with fluorescence lifetime snapshot data and time-resolved high-performance liquid chromatography (HPLC) measurements, snapshot TA reveals that Zea•+ formation is closely related to energy-dependent quenching (qE) in nonphotochemical quenching. Quantitative and dynamic information on CT quenching discussed in this work give insight into the design principles of photoprotection in natural photosynthesis.

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Citations

May 15, 2020·Optics Express·Kelly S WilsonCathy Y Wong
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Soomin ParkGraham R Fleming
Aug 21, 2020·IScience·Francesco SacconTomáš Polívka
Apr 30, 2021·The Plant Journal : for Cell and Molecular Biology·Renata WelcWieslaw I Gruszecki
Jun 26, 2021·Journal of Plant Research·Jeffrey A Cruz, Thomas J Avenson
Dec 19, 2017·The Journal of Physical Chemistry Letters·Bart van OortRoberta Croce
Mar 24, 2018·The Journal of Physical Chemistry Letters·Yusaku HontaniJohn T M Kennis
Jan 1, 2022·Biophysical Journal·Fatemeh Azadi-ChegeniAnjali Pandit

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