SNARE assembly and membrane fusion, a kinetic analysis.

The Journal of Biological Chemistry
Fan ZhangYeon-Kyun Shin

Abstract

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) assembly may promote intracellular membrane fusion, an essential process for vesicular transport in cells. Core complex formation between vesicle-associated SNARE and target membrane SNARE perhaps drives the merging of two membranes into a single bilayer. Using spin-labeling EPR, trans-SNARE complex formation was monitored "locally" at four different core locations of recombinant yeast SNAREs, which are individually reconstituted into phospholipid vesicles. The results indicate that the time scales of core formation are virtually the same at all four locations throughout the core region, indicating the possibility of a single step core assembly, which appears to be somewhat different from what has been postulated by the "zipper" model. The EPR data were then compared with the kinetics of the lipid mixing measured with the fluorescence assay. The analysis suggests that SNARE core assembly occurs on a much faster time scale than the lipid mixing, providing a new insight into the timing of individual events in SNARE-induced membrane fusion.

References

Nov 3, 1994·Nature·J E Rothman
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Feb 14, 1997·The Journal of Biological Chemistry·D FasshauerA T Brünger
Dec 9, 1997·Neuron·R C Lin, R H Scheller
Apr 7, 1998·Cell·T WeberJ E Rothman
Sep 10, 1998·Nature Structural Biology·M A PoirierM K Bennett
Sep 10, 1998·Nature Structural Biology·K L NicholsonF M Hughson
Nov 18, 1998·Current Opinion in Structural Biology·W L HubbellM A Lietzow
Feb 27, 1999·Nature Structural Biology·K M FiebigA T Brunger
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·F ParlatiJ E Rothman
Nov 26, 1999·Nature Neuroscience·S Y Hua, M P Charlton
Jun 29, 2000·Annual Review of Biochemistry·R Jahn, T C Südhof
Aug 31, 2000·Nature Structural Biology·W L HubbellC Altenbach
Oct 14, 2000·Annual Review of Cell and Developmental Biology·R C Lin, R H Scheller
Mar 30, 2001·The Journal of Biological Chemistry·M MargittaiR Langen
Apr 12, 2001·Current Opinion in Structural Biology·A T Brunger
Jan 12, 2002·Nature Structural Biology·Wolfram AntoninThomas R Schneider
Jul 18, 2002·The Journal of Cell Biology·Lara K MahalThomas H Söllner
Aug 3, 2002·Nature Reviews. Neuroscience·Josep Rizo, Thomas C Südhof
Sep 11, 2002·Biochemistry·Chang Sup KimYeon-Kyun Shin
Sep 6, 2002·The Journal of Cell Biology·Thomas J MeliaJames E Rothman
Jan 17, 2003·The Journal of Biological Chemistry·Dae-Hyuk KweonYeon-Kyun Shin
Feb 26, 2003·Cell·Reinhard JahnThomas C Südhof
Aug 14, 2003·Biochimica Et Biophysica Acta·Dirk Fasshauer
Aug 14, 2003·Biochimica Et Biophysica Acta·Jeffrey E Gerst
Oct 8, 2003·Neuropharmacology·Maria F MatosThomas C Südhof
Feb 7, 2004·The EMBO Journal·Yong ChenYeon-Kyun Shin

❮ Previous
Next ❯

Citations

Mar 28, 2006·Nature Structural & Molecular Biology·Akhil BhallaEdwin R Chapman
Mar 17, 2006·Nucleic Acids Research·Christoph A MertenChristian J Buchholz
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Meyer B Jackson, Edwin R Chapman
Jul 6, 2014·Nature Communications·Gustav NordlundChristoph von Ballmoos
Jan 19, 2006·The Journal of Cell Biology·Xue Han, Meyer B Jackson
Dec 21, 2005·The Journal of Cell Biology·Christoph Reese, Andreas Mayer
Jan 24, 2008·Biophysical Journal·Marie-Pierre DurrieuMarc Baaden
Sep 11, 2009·The Biochemical Journal·Matthew A Churchward, Jens R Coorssen
Mar 4, 2009·Biophysical Journal·Jinyoung ChangYeon-Kyun Shin
Dec 17, 2014·Molecular Biology of the Cell·Jessica B A SadlerGwyn W Gould
May 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Hakhamanesh MostafaviBen O'Shaughnessy
Aug 5, 2006·The Journal of Biological Chemistry·Thomas J MeliaJames E Rothman
Feb 17, 2005·The Journal of Biological Chemistry·Yinghui ZhangYeon-Kyun Shin
Nov 19, 2005·Proteomics·Kerrie-Ann McMahonRichard G W Anderson
Jul 1, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter N PushparajAlirio J Melendez
Sep 1, 2005·Journal of Cell Science·Christian Ungermann, Dieter Langosch
Apr 19, 2008·Chemical Reviews·James A McNew

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Dirk FasshauerReinhard Jahn
Nature Structural Biology
Dae-Hyuk KweonYeon-Kyun Shin
© 2022 Meta ULC. All rights reserved