SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus

Frontiers in Molecular Neuroscience
Suleman HussainSvend Davanger

Abstract

Syntaxins are a family of membrane-integrated proteins that are instrumental in exocytosis of vesicles. Syntaxin-1 is an essential component of the presynaptic exocytotic fusion machinery in the brain and interacts with several other proteins. Syntaxin-1 forms a four-helical bundle complex with proteins SNAP-25 and VAMP2 that drives fusion of vesicles with the plasma membrane in the active zone (AZ). Little is known, however, about the ultrastructural localization of syntaxin-1 at the synapse. We have analyzed the intrasynaptic expression of syntaxin-1 in glutamatergic hippocampal synapses in detail by using quantitative postembedding immunogold labeling. Syntaxin-1 was present in highest concentrations at the presynaptic AZ, supporting its role in transmitter release. Presynaptic plasma membrane lateral to the AZ, as well as presynaptic cytoplasmic (PreCy) vesicles were also labeled. However, syntaxin-1 was also significantly expressed in postsynaptic spines, where it was localized at the postsynaptic density (PSD), at postsynaptic lateral membranes and in postsynaptic cytoplasm. Postsynaptically, syntaxin-1 colocalized in the nanometer range with the N-methyl-D-aspartate (NMDA) receptor subunit NR2B, but only weakly with the ...Continue Reading

References

Jan 1, 1984·Journal of Neurochemistry·F Fonnum
Sep 10, 1993·Cell·M K BennettR H Scheller
Feb 3, 1995·The Journal of Biological Chemistry·S M Bajjalieh, R H Scheller
Jun 1, 1994·Current Opinion in Neurobiology·M K Bennett, R H Scheller
Nov 1, 1993·Journal of Neurocytology·S KohY Tashiro
Dec 1, 1996·Molecular Biology of the Cell·S H LowK E Mostov
Dec 1, 1996·Molecular Biology of the Cell·H Y GaisanoW S Trimble
Dec 1, 1996·Cell Structure and Function·F MiyaY Tashiro
Jun 11, 1998·Science·C RosenmundC F Stevens
Feb 20, 1999·The Journal of Biological Chemistry·B YangR H Scheller
May 21, 1999·The Journal of Biological Chemistry·D FasshauerR Jahn
Dec 8, 2007·Handbook of Experimental Pharmacology·Thorsten Lang, Reinhard Jahn
Mar 6, 2009·Cell Adhesion & Migration·Jonathan G Hanley
Nov 26, 2009·The Journal of Comparative Neurology·Núria BesalduchMaria Angel Lanuza
May 18, 2010·Neuron·Karine Pozo, Yukiko Goda
Feb 12, 2013·Neuron·Sandra JuradoRobert C Malenka
Jan 31, 2014·Frontiers in Cellular Neuroscience·Arup R NathElise F Stanley

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Citations

Oct 28, 2018·The European Journal of Neuroscience·Sanjay AryalSvend Davanger
Nov 1, 2018·Brain Structure & Function·S HussainS Davanger
Jan 4, 2019·Journal of Molecular Cell Biology·Rosalba D'Alessandro, Jacopo Meldolesi
Sep 6, 2017·Clinical and Experimental Hypertension : CHE·Peng LiChang-Ping Hu
Jun 15, 2017·Frontiers in Cellular Neuroscience·Farr Niere, Kimberly F Raab-Graham
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yi Gu, Richard L Huganir
Feb 4, 2021·Aging and Disease·Huang KuangShu-Long Yang

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Methods Mentioned

BETA
electron microscopy
light microscopy
confocal microscopy
co-immunoprecipitation

Software Mentioned

SPSS
adobe photoshop
vSNARE

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