SNAREs, HOPS and regulatory lipids control the dynamics of vacuolar actin during homotypic fusion in S. cerevisiae

Journal of Cell Science
Surya KarunakaranRutilio A Fratti

Abstract

Homotypic vacuole fusion requires SNAREs, the Rab Ypt7p, the tethering complex HOPS, regulatory lipids and actin. In Saccharomyces cerevisiae, actin functions at two stages of vacuole fusion. Pre-existing actin filaments are depolymerized to allow docking and assembly of the vertex ring (a microdomain enriched in proteins and lipids that mediate fusion). Actin is then polymerized late in the pathway to aid fusion. Here, we report that the fusion machinery regulates the accumulation of actin at the vertex ring. Using Cy3-labeled yeast actin to track its dynamics, we found that its vertex enrichment was abolished when actin monomers were stabilized by latrunculin-B, independent of the extent of incorporation. By contrast, stabilization of filamentous actin with jasplakinolide markedly augmented actin vertex enrichment. Importantly, agents that inhibit SNAREs, Ypt7p and HOPS inhibited the vertex enrichment of actin, demonstrating that the cytoskeleton and the fusion machinery are interdependently regulated. Actin mobilization was also inhibited by ligating ergosterol and PtdIns(3)P, whereas the ligation or modification of PtdIns(4,5)P(2) augmented the vertex enrichment of actin. The proteins and lipids that regulated actin mobiliz...Continue Reading

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Citations

Jul 2, 2016·Traffic·Matthew L StarrRutilio A Fratti
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Sep 1, 2016·Annual Review of Cell and Developmental Biology·Kay O SchinkHarald Stenmark
Jan 9, 2019·The Journal of Biological Chemistry·Matthew L StarrRutilio A Fratti
Jan 18, 2020·The Biochemical Journal·Herre Jelger Risselada, Andreas Mayer
Jul 29, 2020·Frontiers in Cell and Developmental Biology·Logan R Hurst, Rutilio A Fratti
Sep 12, 2018·International Journal of Molecular Sciences·Michael OgdenGhazanfar Abbas Khan
Dec 28, 2018·Trends in Biochemical Sciences·Matthew L Starr, Rutilio A Fratti

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