SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis

PloS One
Christina MüllerRobin White

Abstract

Myelin basic proteins (MBP) are major constituents of the myelin sheath in the central nervous system (CNS) and the peripheral nervous system (PNS). In the CNS Mbp translation occurs locally at the axon-glial contact site in a neuronal activity-dependent manner. Recently we identified the small non-coding RNA 715 (sncRNA715) as a key inhibitor of Mbp translation during transport in oligodendrocytes. Mbp mRNA localization in Schwann cells has been observed, but has not been investigated in much detail. Here we could confirm translational repression of Mbp mRNA in Schwann cells. We show that sncRNA715 is expressed and its levels correlate inversely with MBP in cultured Schwann cells and in the sciatic nerve in vivo. Furthermore we could reduce MBP protein levels in cultured Schwann cells by increasing the levels of the inhibitory sncRNA715. Our findings suggest similarities in sncRNA715-mediated translational repression of Mbp mRNA in oligodendrocytes and Schwann cells.

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Citations

Jun 28, 2018·Frontiers in Molecular Neuroscience·Julie Torvund-JensenLisbeth S Laursen
Mar 27, 2018·Glia·Kerstin MüllerChristina F Vogelaar

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Methods Mentioned

BETA
PCR
Reverse transcription PCR
transfection
FCS

Software Mentioned

ImageLab
cell
REST
GraphPad Prism5

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