SNIPER peptide-mediated degradation of endogenous proteins

Current Protocols in Chemical Biology
Xuelai Fan, Yu Tian Wang

Abstract

Rapid and reversible methods for altering the function of endogenous proteins are not only indispensable tools for probing complex biological systems, but may potentially drive the development of new therapeutics for the treatment of human diseases. Genetic approaches have provided insights into protein function, but are limited in speed, reversibility and spatiotemporal control. To overcome these limitations, we have developed a peptide-based method (SNIPER: Selective Native Protein Eradication) to degrade any given endogenous protein at the post-translational level by harnessing chaperone-mediated autophagy, a major intracellular protein degradation pathway. This unit presents a typical strategy in the design and validation of a protein-knockdown peptide.

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Citations

Jan 24, 2019·Journal of Fish Biology·Zohar Ibarra-ZatarainNeil Duncan
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Apr 12, 2021·The Journal of Biological Chemistry·Shanique B Alabi, Craig M Crews

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