SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway

Journal of Translational Medicine
Wenjing ZhuMinjie Wei

Abstract

Ovarian cancer is the leading cause of death in gynecological cancer. Cancer stem cells (CSCs) contribute to the occurrence, progression and resistance. Small nucleolar RNAs (SnoRNAs), a class of small molecule non-coding RNA, involve in the cancer cell stemness and tumorigenesis. In this study, we screened out SNORNAs related to ovarian patient's prognosis by analyzing the data of 379 cases of ovarian cancer patients in the TCGA database, and analyzed the difference of SNORNAs expression between OVCAR-3 (OV) sphere-forming (OS) cells and OV cells. After overexpression or knockdown SNORD89, the expression of Nanog, CD44, and CD133 was measured by qRT-PCR or flow cytometry analysis in OV, CAOV-3 (CA) and OS cells, respectively. CCK-8 assays, plate clone formation assay and soft agar colony formation assay were carried out to evaluate the changes of cell proliferation and self-renewal ability. Scratch migration assay and trans-well invasion analysis were used for assessing the changes of migration and invasion ability. High expression of SNORD89 indicates the poor prognosis of ovarian cancer patients and was associated with patients' age, therapy outcome. SNORD89 highly expressed in ovarian cancer stem cells. The overexpression o...Continue Reading

References

Jul 8, 1999·Current Opinion in Cell Biology·L B Weinstein, J A Steitz
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Apostolos KlinakisArgiris Efstratiadis
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Teresa PalomeroAdolfo A Ferrando
Feb 8, 2007·Nucleic Acids Research·Steve L ReichowGabriele Varani
Mar 3, 2007·Cell Cycle·Argiris EfstratiadisApostolos Klinakis
Sep 8, 2009·Nature Medicine·Piyush B GuptaRobert A Weinberg
Jan 7, 2010·The Journal of Clinical Investigation·Natasha Y FrankMarkus H Frank
Mar 17, 2010·Molecular Cell·Tamás KissBeáta E Jády
Jul 3, 2010·Nature·Peter Dirks
Apr 28, 2011·CA: a Cancer Journal for Clinicians·Danijela Jelovac, Deborah K Armstrong
Aug 5, 2011·Drugs·R Wendel Naumann, Robert L Coleman
Apr 24, 2012·Leukemia·W ValleronP Brousset
Jun 26, 2012·Contemporary Nurse·Victoria Jayde, Maureen Boughton
Feb 5, 2013·Cancer Letters·Elena S Martens-UzunovaGuido Jenster
Jul 3, 2013·Molecular Aspects of Medicine·Nuzhat AhmedJock K Findlay
Jun 3, 2014·Molecular Cancer·Kaiissar MannoorFeng Jiang
Jul 18, 2014·OncoTargets and Therapy·Claudia MarchettiPierluigi Benedetti Panici
Jul 31, 2014·Nucleic Acids Research·Gabrielle Deschamps-FrancoeurMichelle S Scott
Aug 28, 2014·International Journal of Cancer. Journal International Du Cancer·Lu GaoFeng Jiang
Sep 15, 2014·Molecular Cancer·Gang XuZhong-Tian Qi
Nov 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Suping ZhangThomas J Kipps
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Amit Kumar SrivastavaQi-En Wang
May 16, 2015·Journal of Experimental & Clinical Cancer Research : CR·Di ZhengGang Chen
Feb 3, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Nicolette G AlkemaSteven de Jong
Feb 11, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Priyanka BhattRajashree Mashru
May 14, 2016·Nucleic Acids Research·Hadi JorjaniAndreas R Gruber
Jan 25, 2017·Blood·Marta Sanchez-Martin, Adolfo Ferrando
Apr 19, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Long WuYufeng Yuan
Apr 30, 2017·Seminars in Cancer Biology·Petronella Beatrix Ottevanger
Jun 27, 2017·Nature Cell Biology·Fengbiao ZhouCarsten Müller-Tidow
Jul 14, 2017·NPJ Breast Cancer·Dillon G PattersonGlen M Borchert

❮ Previous
Next ❯

Citations

Jul 27, 2020·Dose-response : a Publication of International Hormesis Society·Jun-Yan HeZhi-Dong Wang
Oct 25, 2020·Molecular and Cellular Biochemistry·Mohammad Mahdi ForghanifardMohammad Reza Abbaszadegan
Nov 24, 2020·Frontiers in Cell and Developmental Biology·Liwen ZhangMiao He
Jan 6, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Venzil Lavie DsouzaShama Prasada Kabekkodu
Apr 5, 2021·Biochemical and Biophysical Research Communications·Dandan WeiShiqing Jiang
Jun 3, 2021·Non-coding RNA·Laeya BaldiniStéphane Labialle
Jun 29, 2021·Seminars in Cancer Biology·Showket HussainShafiul Haque
Nov 16, 2021·RNA Biology·Daniela Barros-SilvaElena S Martens-Uzunova

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
PCR
electrophoresis
Flow Cytometry
transfection
chips
chip
reverse transcription PCR

Software Mentioned

GraphPad Prism
GraphPad prism7
SPSS Statistics
NovoExpress
Image J
Edge R

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Xinling ZhouMin Wang
International Journal of Clinical and Experimental Medicine
Yueyin Pan, Xudong Huang
© 2021 Meta ULC. All rights reserved