Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p.

Molecular and Cellular Biology
H R ChenS C Cheng

Abstract

The yeast protein Prp19p is essential for pre-mRNA splicing and is associated with the spliceosome concurrently with or just after dissociation of U4 small nuclear RNA. In splicing extracts, Prp19p is associated with several other proteins in a large protein complex of unknown function, but at least one of these proteins is also essential for splicing (W.-Y. Tarn, C.-H. Hsu, K.-T. Huang, H.-R. Chen, H.-Y. Kao, K.-R. Lee, and S.-C. Cheng, EMBO J. 13:2421-2431, 1994). To identify proteins in the Prp19p-associated complex, we have isolated trans-acting mutations that exacerbate the phenotypes of conditional alleles of prp19, using the ade2-ade3 sectoring system. A novel splicing factor, Snt309p, was identified through such a screen. Although the SNT309 gene was not essential for growth of Saccharomyces cerevisiae under normal conditions, yeast cells containing a null allele of the SNT309 gene were temperature sensitive and accumulated pre-mRNA at the nonpermissive temperature. Far-Western blot analysis revealed direct interaction between Prp19p and Snt309p. Snt309p was shown to be a component of the Prp19p-associated complex by Western blot analysis. Immunoprecipitation studies demonstrated that Snt309p was also a spliceosomal com...Continue Reading

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Citations

Aug 22, 2015·Science·Chuangye YanYigong Shi
Jan 24, 2002·Molecular Cell·Scott W StevensJohn Abelson
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·E L PerkinsM A Resnick
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·H R ChenS C Cheng
Dec 2, 2004·Biochimica Et Biophysica Acta·Cecile M Pickart, Michael J Eddins
Oct 9, 2014·Nucleic Acids Research·Hsin-Chou ChenSoo-Chen Cheng
Dec 21, 2016·Molecular and Cellular Biology·Nan-Ying WuSoo-Chen Cheng
Mar 11, 2003·Nature Structural Biology·Melanie D OhiKathleen L Gould
Mar 30, 2012·Transcription·Sittinan ChanaratKatja Sträßer
Jul 6, 2012·Bioscience Reports·Hsin-Chou Chen, Soo-Chen Cheng
Mar 27, 1999·The Journal of Biological Chemistry·W Y TsaiS C Cheng
Aug 26, 2009·Molecular and Cellular Biology·Ying-Fang ChiuSoo-Chen Cheng
Feb 24, 2010·Molecular and Cellular Biology·Michael GroteReinhard Lührmann
Jun 27, 2002·Biochimica Et Biophysica Acta·Seyung ChungBrian C Rymond
Dec 17, 2004·Molecular and Cellular Biology·Melanie D OhiKathleen L Gould
Sep 13, 2003·Science·Shih-Peng ChanSoo-Chen Cheng
Dec 17, 2005·Genes & Development·Rong-Tzong TsaiSoo-Chen Cheng

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