SOCS3 silencing attenuates eosinophil functions in asthma patients

International Journal of Molecular Sciences
M Paz ZafraVictoria del Pozo

Abstract

Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high levels of suppressor of cytokine signaling 3 (SOCS3). In this article, SOCS3 gene silencing in eosinophils from asthmatics has been carried out to achieve a better understanding of the suppressor function in eosinophils. SOCS3 siRNA treatment drastically reduced SOCS3 expression in eosinophils, leading to an inhibition of the regulatory transcription factors GATA-3 and FoxP3, also interleukin (IL)-10; in turn, an increased STAT3 phosphorilation was observed. Moreover, SOCS3 abrogation in eosinophils produced impaired migration, adhesion and degranulation. Therefore, SOCS3 might be regarded as an important regulator implicated in eosinophil mobilization from the bone marrow to the lungs during the asthmatic process.

References

Jan 1, 1994·International Archives of Allergy and Immunology·S TakafujiC A Dahinden
Apr 23, 1999·The Journal of Biological Chemistry·N ZimmermannM E Rothenberg
May 4, 1999·International Archives of Allergy and Immunology·G WoerlyM Capron
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles E EgwuaguIgal Gery
Apr 26, 2003·DNA and Cell Biology·Sun-Hee ParkTae Yoon Kim
Jul 9, 2003·Nature Medicine·Yoh-ichi SekiMasato Kubo
Jul 25, 2003·Pulmonary Pharmacology & Therapeutics·M H GascoigneN Gozzard
Jul 28, 2004·Trends in Immunology·Joanne Elliott, James A Johnston
Feb 16, 2006·Current Allergy and Asthma Reports·Masato Kubo, Hiromasa Inoue
Mar 23, 2006·Annual Review of Immunology·Marc E Rothenberg, Simon P Hogan
Oct 10, 2006·The Journal of Biological Chemistry·Yang LuGiichi Takaesu
Feb 3, 2007·Current Medicinal Chemistry·Hiromasa InoueAkihiko Yoshimura
May 8, 2007·The Journal of Allergy and Clinical Immunology·Helene F RosenbergPaul S Foster
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicholas GoplenRafeul Alam
Jun 17, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stefania MadonnaCristina Albanesi
Aug 30, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P AkuthotaP F Weller
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva M SturmAkos Heinemann
Jun 2, 2009·American Journal of Respiratory and Critical Care Medicine·Prescott G WoodruffJohn V Fahy
Oct 8, 2009·Nature Reviews. Immunology·Jeffrey A BluestoneBrigitta Stockinger
May 29, 2010·American Journal of Respiratory Cell and Molecular Biology·Atsushi MoriwakiYoichi Nakanishi
Jul 19, 2011·Clinical & Developmental Immunology·Esther LópezVictoria Del Pozo
Jul 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xingxia LiuRobert C H Zhao
Aug 22, 2012·The Clinical Respiratory Journal·Jørgen VestboRoberto Rodriguez-Roisin
Nov 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jiyoung OhChoong-Eun Lee
Feb 23, 2013·Critical Reviews in Immunology·Charles D Mills
Jul 23, 2013·The Journal of Allergy and Clinical Immunology·Michel LavioletteNestor A Molfino
Oct 9, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cheng-Rong YuCharles E Egwuagu
Oct 19, 2013·Stroke; a Journal of Cerebral Circulation·María Isabel CuarteroMaría A Moro
Mar 7, 2014·Frontiers in Immunology·Berit Carow, Martin E Rottenberg
May 23, 2014·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Trung N TranDavid Gossage

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Citations

Feb 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christina DraijerMarc Peters-Golden
Oct 1, 2019·Mediators of Inflammation·Fernanda P R SantanaCarla M Prado
Aug 28, 2021·International Journal of Molecular Sciences·Émile Bélanger, Catherine Laprise

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Methods Mentioned

BETA
PCR
confocal microscopy
flow cytometry
transfection
density gradient centrifugation
fluorescence microscopy

Software Mentioned

Image J

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