Abstract
We here investigated the effects of overexpressed superoxide dismutase (SOD)1 and amyotrophic lateral sclerosis (ALS)-linked SOD1 mutants G93A and G147S in Neuro 2A (N2A) cell lines, and found a three-fold increase in lamellipodia either in cells cultured under differentiated or undifferentiated growth conditions. In undifferentiated N2A cells, SOD1 constructs promoted lamellipodial protrusions to similar extent as the overexpression of Rac1, and SOD1-mediated lamellipodia were prevented by coexpression of the N17 dominant-negative form of Rac1, or shRNA for a downstream effector of Rac1, the insulin receptor tyrosine kinase substrate p53 (IRSp53) or its binding partner LIN7. Moreover, no additive effect was measured by coexpression of the SOD1 constructs with Rac1, IRSp53 or LIN7. Collectively these data support a role for SOD1 in the regulation of Rac1-mediated lamellipodia pathway, a property fully retained by the two SOD1 mutants.
References
Apr 8, 1998·Journal of Neurocytology·G WuR W Ledeen
Dec 29, 2000·Nature·H MikiT Takenawa
Sep 5, 2002·Nature Reviews. Neuroscience·Jorge Santos da Silva, Carlos G Dotti
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joan Selverstone Valentine, P John Hart
Oct 4, 2003·Science·A M ClementD W Cleveland
Nov 5, 2003·FEBS Letters·Kei HoriKenji Sobue
Dec 24, 2003·Journal of Neurochemistry·Massimo TortaroloMarcus Rattray
Jan 28, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeonghoon ChoiEunjoon Kim
Jun 3, 2006·Science·Séverine BoilléeDon W Cleveland
Feb 5, 2008·Nature Neuroscience·Koji YamanakaDon W Cleveland
Apr 9, 2008·The Journal of Cell Biology·Paolo RonchiNica Borgese
May 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Koji YamanakaLawrence S B Goldstein
Dec 5, 2008·Traffic·Silvia MassariGrazia Pietrini
Apr 20, 2010·Neurobiology of Disease·Stella GagliardiCristina Cereda
Aug 11, 2011·Human Molecular Genetics·Maria Grazia PesaresiMauro Cozzolino
Sep 21, 2011·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Paola OrigonePaola Mandich
Jul 7, 2012·Journal of Cell Science·Arianna CrespiGrazia Pietrini
Mar 7, 2013·Nature Reviews. Neuroscience·Wim Robberecht, Thomas Philips
May 4, 2013·Current Opinion in Cell Biology·Sara BisiGiorgio Scita
Dec 27, 2013·Nature Neuroscience·Alan E RentonBryan J Traynor
Jun 21, 2016·European Journal of Cell Biology·Ilaria FerrariGrazia Pietrini
Apr 21, 2017·Frontiers in Neuroanatomy·Pegah AfsharHassan Marzban