SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells

Oxidative Medicine and Cellular Longevity
Ji JiaTingting Huo

Abstract

Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue. However, whether amifostine could protect neuronal cells against oxidative injury has not been reported. The purpose of this study is to explore the protection of amifostine in PC12 cells. PC12 cells exposed to glutamate were used to mimic neuronal oxidative injury. SOD assay kit was taken to evaluate intracellular Cu/Zn SOD (SOD1) and SOD2 activities; western blot analysis and immunofluorescence staining were performed to investigate SOD2 protein expression; MTT, lactate dehydrogenase (LDH), release and cell morphology were used to evaluate cell injury degree, and apoptotic rate and cleaved caspase-3 expression were taken to assess apoptosis; mitochondrial superoxide production, intracellular reactive oxygen species (ROS), and glutathione (GSH) and catalase (CAT) levels were evaluated by reagent kits. Amifostine increased SOD2 activity and expression, decreased cell injury and apoptosis, reduced mitochondrial superoxide production and intracellular ROS generation, and restored intracellular GSH and CAT levels in PC12 cells exposed to glutamate. SOD2-siRNA, however, sign...Continue Reading

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Citations

Oct 1, 2017·Experimental and Therapeutic Medicine·Runxiao LvZhicai Shi
Mar 22, 2019·International Journal of Molecular Medicine·Hae Jin LeeSeung Hwan Yang
Oct 1, 2020·The Journal of International Medical Research·Chengli LingYuhong Wang
Oct 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yuqing WangShuai Yang
Jun 7, 2019·International Journal of Molecular Sciences·Victor CamberosMary Kearns-Jonker
Apr 11, 2017·Oxidative Medicine and Cellular Longevity·Shao-Ze WuKang-Ting Ji

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Methods Mentioned

BETA
Assay
confocal microscopy
flow cytometry
transfection

Software Mentioned

image pro - plus
SPSS
MitoSOX

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