Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice

Scientific Reports
Daniël O J ReijntjesSonja J Pyott

Abstract

Potassium (K+) channels shape the response properties of neurons. Although enormous progress has been made to characterize K+ channels in the primary auditory neurons, the molecular identities of many of these channels and their contributions to hearing in vivo remain unknown. Using a combination of RNA sequencing and single molecule fluorescent in situ hybridization, we localized expression of transcripts encoding the sodium-activated potassium channels KNa1.1 (SLO2.2/Slack) and KNa1.2 (SLO2.1/Slick) to the primary auditory neurons (spiral ganglion neurons, SGNs). To examine the contribution of these channels to function of the SGNs in vivo, we measured auditory brainstem responses in KNa1.1/1.2 double knockout (DKO) mice. Although auditory brainstem response (wave I) thresholds were not altered, the amplitudes of suprathreshold responses were reduced in DKO mice. This reduction in amplitude occurred despite normal numbers and molecular architecture of the SGNs and their synapses with the inner hair cells. Patch clamp electrophysiology of SGNs isolated from DKO mice displayed altered membrane properties, including reduced action potential thresholds and amplitudes. These findings show that KNa1 channel activity is essential fo...Continue Reading

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Citations

Mar 2, 2021·The Journal of Physiology·Mark A RutherfordJuan D Goutman
Apr 6, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rebekka EhingerRobert Lukowski
Jun 18, 2021·European Journal of Human Genetics : EJHG·Thashi BharadwajSuzanne M Leal
Jun 3, 2021·Trends in Pharmacological Sciences·Bethan A ColeJonathan D Lippiat

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Methods Mentioned

BETA
RNAseq
smFISH
electrophoresis
light microscopy
confocal microscopy

Software Mentioned

Illustrator
RNAseq
HTSeq
ImageJ
Imaris
hisat
Image J
Adobe PhotoShop
smFISH
LAS X

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