PMID: 39251Jul 1, 1979

Sodium azide-induced mutagenesis in Saccharomyces cerevisiae

Mutation Research
L SilhánkováJ Velemínský

Abstract

Sodium azide (0.5--2.0 X 10(-5) M), applied for 24 h on cells growing in complete medium, increased up to 26 times the frequency of reversions and locus-specific suppressor mutations of allele ilv1-92 in diploid strain D7 of Saccharomyces cerevisiae. Similarly, it enhanced the frequency of reversions and/or mitotic gene conversions of alleles trp5-12/trp5-27 up to 19 times. Reconstruction experiments showed that the increase of mutations in complete medium was not due to a selection of prototrophic types under growth conditions and, therefore, that sodium azide acts as a weak mutagen in S. cerevisiae under growth conditions at a low pH. No mutagenic or convertogenic effect was observed when azide was applied to resting cells in buffer at pH 4.2.

References

Jul 1, 1979·Mutation Research·J VelemínskýT Gichner
Feb 1, 1988·Mutation Research·W M Owais, A Kleinhofs
Dec 1, 1982·Regulatory Toxicology and Pharmacology : RTP·K A Frederick, J G Babish

Citations

Jan 1, 1977·Mutation Research·J VelemínskýV Pokorný
Jul 1, 1979·Mutation Research·J VelemínskýT Gichner
Dec 1, 1976·Mutation Research·A Kleinhofs, J A Smith
Sep 1, 1975·Mutation Research·A KleinhofsS Von Broembsen
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Oct 1, 1973·Mutation Research·E Gundelach

Related Concepts

ILV1
Saccharomyces cerevisiae
Hydrogen-Ion Concentration
Clastogens
TRP5
Azides

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