Sodium butyrate inhibits the phosphorylation of the retinoblastoma gene product in mouse fibroblasts by a transcription-dependent mechanism

Journal of Cellular Physiology
C Buquet-FagotJ Mester

Abstract

In the chemically transformed mouse fibroblasts BP-A31, the retinoblastoma protein (pRB) is hypophosphorylated at quiescence and becomes hyperphosphorylated after approximately 6 h of serum stimulation. Phosphorylation of pRb was blocked if sodium butyrate was added together with serum or within 3 h afterwards. Actinomycin D added 3 h after serum stimulation did not prevent pRb phosphorylation, but it reversed the inhibitory effect of butyrate. These observations indicate that sodium butyrate acts by turning on the expression of gene(s) coding for proteins which prevent the accumulation of hyperphosphorylated pRb. Such butyrate-induced inhibitor(s) may interfere with the phosphorylation of pRb by cyclin-dependent kinases. Treatment of quiescent BP-A31 cells with serum in the presence of sodium butyrate has led to an increased cell content of the Waf1/CIP1 mRNA (coding for a cyclin-dependent) kinase inhibitory protein) compared with serum alone, suggesting a possible role of p21Waf1/CIP1. In contrast, the mitogen activated protein kinase (enzyme which has been shown to phosphorylate pRb) was constitutively active in BP-A31 cells, and its activity was not significantly affected by a < or = 3h incubation with sodium butyrate.

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Citations

Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Nathalie VillerbuJan Mester
Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·Y TeraoN Wake
Feb 7, 2001·Molecular and Cellular Biology·Y C ChangN H Heintz
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·M FiscellaE Appella
Apr 7, 2005·Biotechnology and Bioengineering·Pedro Lei, Stelios T Andreadis
Mar 5, 2004·International Journal of Cancer. Journal International Du Cancer·Monique BedinJan Mester
Dec 2, 2005·Life Sciences·Kathleen M GilbertE Kim Fifer
Jan 27, 1999·Biochemical and Biophysical Research Communications·B Chénais
May 13, 2003·The Proceedings of the Nutrition Society·Hervé M BlottièreChristine Cherbut
Apr 5, 2000·The Journal of Biological Chemistry·D CourilleauJ Mester
Mar 12, 2002·Archives of Pharmacal Research·Adorjan Aszalos
Jan 1, 1997·Pathology Oncology Research : POR·Adorján Aszalós, Sándor Eckhardt
Jun 2, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Md Ruhul AminJaleh Malakooti
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S K JacksonK M Gilbert

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