Sodium butyrate stimulates mineralized nodule formation and osteoprotegerin expression by human osteoblasts

Archives of Oral Biology
Tomoko KatonoMasao Maeno

Abstract

Butyric acid (sodium butyrate; BA) is a major metabolic by-product of main periodontopathic bacteria present in subgingival plaque. In the present study, we examined the effects of BA on cell proliferation, alkaline phosphatase (ALPase) activity, mineralized nodule formation, extracellular matrix protein expression, macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) in normal human osteoblasts. The cells were cultured with 0, 10(-8), 10(-6) or 10(-4)M BA for up to 12 days. Mineralized nodule formation was detected by alizarin red staining, and the calcium content in mineralized nodules was determined using a calcium assay kit. The gene and protein expression levels for type I collagen, bone sialoprotein (BSP), osteopontin (OPN), M-CSF, and OPG were examined using real-time PCR and ELISA, respectively. Mineralized nodule formation and the calcium content of mineralized nodules were increased by BA in a dose-dependent manner. Cell proliferation and ALPase activity were not affected by the addition of BA. Following the addition of 10(-4)M BA, the expression levels of BSP, OPN, and OPG increased, whereas the expression levels of type I collagen and M-CSF were not markedly affected. These results suggest that BA...Continue Reading

References

Sep 9, 1992·International Journal of Cancer. Journal International Du Cancer·L GametJ C Murat
Jan 1, 1990·The Journal of Infectious Diseases·C EftimiadiG A Rossi
Sep 1, 1985·Journal of Periodontal Research·G A BottaG Blasi
Jan 1, 1980·Calcified Tissue International·D C WilliamsC C Johnston
Nov 1, 1993·The Journal of Experimental Medicine·K FullerT J Chambers
Dec 1, 1993·Scandinavian Journal of Dental Research·P O SöderB Söder
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G K Hunter, H A Goldberg
Jan 1, 1997·International Journal of Systematic Bacteriology·T UmemotoI Namikawa
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R NiedermanS Kashket
Oct 6, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J SodekM D McKee
Apr 16, 2002·Infection and Immunity·Tomoko Kurita-OchiaiKazuo Fukushima
Jul 11, 2002·Oral Diseases·T Katagiri, N Takahashi
May 27, 2006·Molecular Endocrinology·Hyun Woo LeeJae Bum Kim
Mar 3, 2007·Biochemical and Biophysical Research Communications·Tain-Hsiung ChenShih-Chieh Hung

❮ Previous
Next ❯

Citations

Nov 13, 2012·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·H-B HsiaoW-C Lin
Sep 7, 2012·The British Journal of Nutrition·Li-Chan YangWen-Chuan Lin
Jul 1, 2010·Journal of Oral Science·Li YangYorimasa Ogata
Feb 12, 2011·Journal of Tissue Engineering and Regenerative Medicine·Kevin W-H LoCato T Laurencin
Sep 30, 2017·Gut Microbes·Jing YanJulia F Charles
Jan 24, 2019·Oral Diseases·Ming JiangGuangxun Zhu
Jul 16, 2019·The Journal of Clinical Investigation·Mario M ZaissRoberto Pacifici
Oct 4, 2017·The Journal of Clinical Endocrinology and Metabolism·Yuan-Cheng ChenHong-Wen Deng
Dec 5, 2019·Cell Proliferation·Minyue BaoLing Ye
Jun 11, 2017·Scientific Reports·Emilie NormandValérie Marcil
Apr 15, 2020·Current Osteoporosis Reports·René Rizzoli, Emmanuel Biver
Feb 3, 2019·Aging Clinical and Experimental Research·René Rizzoli
May 7, 2020·Frontiers in Cellular and Infection Microbiology·Zhiming XuGuofu Pi
Apr 23, 2021·Journal of Leukocyte Biology·Ye TuXuedong Zhou
May 25, 2021·Current Opinion in Nephrology and Hypertension·Elizabeth KermgardKatherine Wesseling-Perry

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Tania M Schroeder, Jennifer J Westendorf
Clinica Chimica Acta; International Journal of Clinical Chemistry
P DanielC Chany
Bioscience, Biotechnology, and Biochemistry
Rieko MitamuraYoritaka Aoyama
© 2022 Meta ULC. All rights reserved