PMID: 3214178Dec 1, 1988Paper

Sodium-calcium exchange in membrane vesicles from Artemia

Archives of Biochemistry and Biophysics
J Cheon, J P Reeves

Abstract

Membrane vesicles were prepared from Artemia nauplii (San Francisco Bay variety) 45 h after hydration of the dry cysts. Na+-loaded vesicles accumulated up to 10 nmol Ca2+/mg protein when diluted 50-fold into 160 mM KCl containing 15 microM CaCl2. Practically no accumulation of Ca2+ was observed if the vesicles were diluted into 160 mM NaCl instead of KCl, or if they were treated with monensin, a Na+ ionophore, for 30 s prior to addition of CaCl2 to the KCl medium. These observations indicate that the Artemia vesicles exhibit Na-Ca exchange activity. The velocity of Ca2+ accumulation by the vesicles in KCl was stimulated 2.6-fold by the K+ ionophore valinomycin, suggesting that the exchange system is electrogenic, with a stoichiometry greater than 2Na+ per Ca2+. Km,Ca and Vmax values were 15 microM and 7.5 nmol/mg protein.s, respectively. Exchange activity in the Artemia vesicles was inhibited by benzamil (IC50 approximately equal to 100 microM) and by quinacrine (IC50 approximately equal to 250 microM), agents that also inhibit exchange activity in cardiac sarcolemmal vesicles. Unlike cardiac vesicles, however, exchange activity in Artemia was not stimulated by limited proteolysis, redox reagents, or intravesicular Ca2+. This i...Continue Reading

References

Nov 2, 1987·Biochimica Et Biophysica Acta·R S SlaughterD W Morgan
Jan 1, 1987·The American Journal of Physiology·J P Reeves, P Poronnik
Jan 1, 1987·The American Journal of Physiology·P de la Peña, J P Reeves
Sep 1, 1982·The American Journal of Physiology·K D Philipson, A Y Nishimoto
May 16, 1983·European Journal of Biochemistry·P Caroni, E Carafoli
Jan 1, 1984·Progress in Nucleic Acid Research and Molecular Biology·A J Wahba, C L Woodley
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C C HaleJ P Reeves

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Citations

Feb 1, 1991·Archives of Environmental Contamination and Toxicology·T H MacRae, A S Pandey
Nov 7, 1989·European Journal of Pharmacology·L Yang, M Vassalle
Jan 1, 1988·Progress in Biophysics and Molecular Biology·D W Hilgemann
Jan 31, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Michele G WheatlyAdrian M Corbett
Feb 1, 1991·Ecotoxicology and Environmental Safety·A S Pandey, T H MacRae
Feb 1, 1992·The American Journal of Physiology·G F TibbitsH Kashihara
Jun 1, 1989·The American Journal of Physiology·R VemuriK D Philipson

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