Sodium channel distribution within the rabbit atrioventricular node as analysed by confocal microscopy

The Journal of Physiology
K PetreccaA Shrier

Abstract

1. Paired 20 microns thick sections of fresh frozen tissue taken from the frontal plane of the rabbit atrioventricular (AV) nodal region were processed for histology and immunohistochemistry. Confocal microscopy was used to image the distribution of sodium channels using IgG (R12) developed against a highly conserved sequence in the interdomain 3-4 region of cloned sodium channels. 2. In ventricular and atrial cells, sodium channel immunofluorescence was localized to lateral membranes and T-tubules. In the open AV node, levels of sodium channel immunofluorescence in the transitional cell zone and in the lower nodal cell tract were comparable to that found in the atrial and ventricular myocardium. 3. In the enclosed AV node a gradation of sodium channel immunofluorescence is present such that peripherally located circumferential transitional cells display high levels of immunofluorescence, comparable to that of atrial and ventricular myocardium, while centrally located midnodal cells display decreased levels of or no immunofluorescence. 4. In order to correlate the distribution of sodium channels with the distribution of gap junctions, we used IgG directed against the carboxyl terminus of connexin43 (CT-360). Ventricular cell im...Continue Reading

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Citations

Jun 1, 2005·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Michelle S C KhooMark E Anderson
Jun 5, 2002·Journal of Neuroscience Methods·Andre R MassensiniMarco A Romano-Silva
Oct 30, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xianming LinRichard D Veenstra
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·G Alex PapadatosAndrew A Grace
Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sebastian K G MaierTodd Scheuer
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Oct 28, 2008·Journal of Molecular and Cellular Cardiology·Hongwei ChengJules C Hancox
Jul 11, 2014·Frontiers in Physiology·Ashley M NisbetGodfrey L Smith
Mar 27, 2004·Physiological Reviews·André G Kléber, Yoram Rudy
Sep 16, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Igor R EfimovMark Boyett
Aug 30, 2003·Circulation Research·Xianming LinRichard D Veenstra
Oct 18, 2003·Circulation Research·Halina DobrzynskiIgor R Efimov
Sep 27, 2005·Physiological Reviews·Jeanne M Nerbonne, Robert S Kass
Jul 16, 2008·Physiological Reviews·Matteo E Mangoni, Joël Nargeot
Aug 3, 2017·Journal of Arrhythmia·Shin InadaKazuo Nakazawa PhD
Oct 24, 2002·Journal of Molecular and Cellular Cardiology·Gui-Rong LiAlvin Shrier

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